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Calretinin Antibody (CALB2/2602) [CoraFluor™ 1]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, MA
Clone
CALB2/2602
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-08323AF350
Alexa Fluor 405
NBP3-08323AF405
Alexa Fluor 488
NBP3-08323AF488
Alexa Fluor 532
NBP3-08323AF532
Alexa Fluor 594
NBP3-08323AF594
Alexa Fluor 647
NBP3-08323AF647
Alexa Fluor 700
NBP3-08323AF700
Alexa Fluor 750
NBP3-08323AF750
Allophycocyanin
NBP3-08323APC
Biotin
NBP3-08323B
DyLight 350
NBP3-08323UV
DyLight 405
NBP3-08323V
DyLight 488
NBP3-08323G
DyLight 550
NBP3-08323R
DyLight 594
NBP3-08323DL594
DyLight 650
NBP3-08323C
DyLight 680
NBP3-08323FR
DyLight 755
NBP3-08323IR
FITC
NBP3-08323F
HRP
NBP3-08323H
Janelia Fluor 525
NBP3-08323JF525
Janelia Fluor 549
NBP3-08323JF549
Janelia Fluor 585
NBP3-08323JF585
Janelia Fluor 635
NBP3-08323JF635
Janelia Fluor 646
NBP3-08323JF646
Janelia Fluor 669
NBP3-08323JF669
PE
NBP3-08323PE
PerCP
NBP3-08323PCP
Unconjugated
NBP3-07365-100ug
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP3-07365-100ug
Azide and BSA Free
NBP3-08323
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Calretinin Antibody (CALB2/2602) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Recombinant human Calretinin protein fragment (around aa23-242) (exact sequence is proprietary) (Uniprot: P22676)
Localization
Cytoplasmic and Nuclear
Marker
Mesothelioma Marker
Specificity
It recognizes a protein of about 29kDa, which is identified as Calretinin (also known as Calbindin 2). Calretinin is a vitamin D-dependent calcium-binding protein involved in calcium signaling. It is present in subsets of neurons throughout the brain and spinal chord, including sensory ganglia. Antibody to calretinin is useful in differentiating mesothelioma from adenocarcinomas of the lung. It also aids in differentiating adrenal cortical neoplasms from pheochromocytomas.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CALB2
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin
  • Protein Array
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Calretinin Antibody (CALB2/2602) [CoraFluor™ 1]

  • 29 kDa calbindin
  • CAB29
  • CAL2
  • CALB2
  • calbindin 2
  • calbindin 2, (29kD, calretinin)
  • calbindin 2, 29kDa (calretinin)
  • calbindin D29K
  • Calretinin
  • CR

Background

Calretinin (CAB29, calbindin 2) is a calcium binding protein that belongs to calbindin family. It is mainly found in neuronal tissue, high expressed in auditory neurons. It has been implicated in as a calcium buffer, calcium sensor, and as a regulator of apoptosis (1). Calretinin is considered as a specific marker of mesothelllal cells; it is the only marker that has the utility in distinguishing between sarcomatoid mesotheliomas and sarcomatoid renal cell carcinomas (2). It is widely used in the panel of makers for distinguishing mesothlioma from adenocarcinoma. Along with calretinin, the panel includes E-cadherin, CEA, and cytokeratin 5/6 (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Calretinin
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NBP3-08323CL1
Calretinin Antibody (CALB2/2602) [CoraFluor™ 1]
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Bioinformatics

Gene Symbol CALB2