![Immunohistochemistry: Calponin 1 Antibody (2H1) [NBP3-26649] - Image of Calponin 1 Antibody (2H1) diluted at 1:105 and staining in paraffin-embedded human heart tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26649_rabbit-calponin-1-mab-2h1-262202415253315.jpg "Immunohistochemistry: Calponin 1 Antibody (2H1) [NBP3-26649] - Image of Calponin 1 Antibody (2H1) diluted at 1:105 and staining in paraffin-embedded human heart tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clone | 2H1 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Additional Information | Recombinant monoclonal antibody expressed in HEK293F cells |
| Immunogen | A synthesized peptide derived from Human Calponin 1 [UniProt P51911] |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | CNN1 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at -20 to -70C. Avoid freeze-thaw cycles. |
| Buffer | PBS, pH 7.4, 150mM NaCl and 50% glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
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![Immunohistochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunohistochemistry_23317.jpg)
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | CNN1 |
![Western Blot: Calponin 1 Antibody (2H1) [NBP3-26649] - Positive Western Blot detected in: 293 whole cell lysate, Mouse lung tissue. All lanes: Calponin 1 Antibody at 1.05ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 34, 32 KDa Observed band size: 34 KDa](http://images.novusbio.com/fullsize/nbp3-26649_rabbit-calponin-1-mab-2h1-282202411413618.jpg "Western Blot: Calponin 1 Antibody (2H1) [NBP3-26649] - Positive Western Blot detected in: 293 whole cell lysate, Mouse lung tissue. All lanes: Calponin 1 Antibody at 1.05ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 34, 32 KDa Observed band size: 34 KDa")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")