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Calponin 1 Antibody (1H5B5) - BSA Free

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Western Blot: Calponin 1 Antibody (1H5B5) [NBP2-52445] - Analysis using CNN1 mAb against human CNN1 (AA: 16-165) recombinant protein. (Expected MW is 43.1 kDa)
Western Blot: Calponin 1 Antibody (1H5B5) [NBP2-52445] - Analysis using CNN1 mAb against human CNN1 (AA: 16-165) recombinant protein. (Expected MW is 43.1 kDa)
Immunohistochemistry-Paraffin: Calponin 1 Antibody (1H5B5) [NBP2-52445] - Analysis of paraffin-embedded cervical cancer tissues using CNN1 mouse mAb with DAB staining.
Western Blot: Calponin 1 Antibody (1H5B5) [NBP2-52445] - Analysis using CNN1 mAb against HEK293 (1) and CNN1 (AA: 16-165)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry: Calponin 1 Antibody (1H5B5) [NBP2-52445] - Analysis of paraffin-embedded esophageal cancer tissues using CNN1 mouse mAb with DAB staining.
ELISA: Calponin 1 Antibody (1H5B5) [NBP2-52445] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clone
1H5B5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP2-52445-0.025mg
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Calponin 1 Antibody (1H5B5) - BSA Free Summary

Immunogen
Purified recombinant fragment of human Calponin 1 (AA: 16-165) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CNN1
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500-1:2000
Theoretical MW
33.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Calponin 1 Antibody (1H5B5) - BSA Free

  • Basic calponin
  • Calponin 1
  • calponin 1, basic, smooth muscle
  • Calponin H1
  • Calponin H1, smooth muscle
  • calponins, basic
  • CNN1
  • Sm-Calp
  • SMCC
  • SMCCcalponin-1

Background

Calponin is a smooth muscle specific, actin-, tropomyosin- and calmodulin-binding protein thought to be involved in regulation of actomyosin as well as the regulation or modulation of contraction (1). Calponin binds filamentous actin (F-actin) through two distinct sites ABS1 and ABS2, with an affinity in the low micromolar range (2) Immunoreactivity for calponin, along with alpha-smooth muscle actin and smooth muscle myosin heavy chains, confirms the known neoplastic myoepithelial component of adenoid cystic carcinomas and epithelial-myoepithelial carcinomas. The consistently positive staining pattern in adenoid cystic carcinomas may be diagnostically useful in discriminating histologically similar but consistently negative polymorphous low-grade adenocarcinomas (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CNN1