![Western Blot: Calmodulin 2 Antibody (J4D8) - BSA Free [NBP3-21504] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with NBP3-21504 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: NIH3T3 cell lysate. Lane 2: HeLa cell lysate. Lane 3: Jurkat cell lysate. Lane 4: A549 cell lysate. Lane 5: MCF7 cell lysate. Lane 6: Ramos cell lysate. Lane 7: PC3 cell lysate](http://images.novusbio.com/fullsize/nbp3-21504_mouse-calmodulin-2-mab-j4d8-bsa-free-572023142602.jpg "Western Blot: Calmodulin 2 Antibody (J4D8) - BSA Free [NBP3-21504] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with NBP3-21504 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: NIH3T3 cell lysate. Lane 2: HeLa cell lysate. Lane 3: Jurkat cell lysate. Lane 4: A549 cell lysate. Lane 5: MCF7 cell lysate. Lane 6: Ramos cell lysate. Lane 7: PC3 cell lysate")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF |
| Clone | J4D8 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Recombinant human Calmodulin (1-149aa) purified from E. coli (Accesion #: NP_001734; Uniprot #: P0DP24). |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CALM2 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.4), 10% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Calmodulin 2 Antibody (NBP3-21504)Find related products by research area.
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| Gene Symbol | CALM2 |
![Immunocytochemistry/Immunofluorescence: Calmodulin 2 Antibody (J4D8) - BSA Free [NBP3-21504] - ICC/IF analysis of Calmodulin 2 in HeLa cells. The cell was stained with NBP3-21504 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).](http://images.novusbio.com/fullsize/nbp3-21504_mouse-calmodulin-2-mab-j4d8-bsa-free-572023142603.jpg "Immunocytochemistry/Immunofluorescence: Calmodulin 2 Antibody (J4D8) - BSA Free [NBP3-21504] - ICC/IF analysis of Calmodulin 2 in HeLa cells. The cell was stained with NBP3-21504 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).")
![Flow Cytometry: Calmodulin 2 Antibody (J4D8) - BSA Free [NBP3-21504] - Flow cytometry analysis of Calmodulin 2 in HeLa cells. The cell was stained with NBP3-21504 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).](http://images.novusbio.com/fullsize/nbp3-21504_mouse-calmodulin-2-mab-j4d8-bsa-free-572023142600.jpg "Flow Cytometry: Calmodulin 2 Antibody (J4D8) - BSA Free [NBP3-21504] - Flow cytometry analysis of Calmodulin 2 in HeLa cells. The cell was stained with NBP3-21504 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).")
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