![Western Blot: Calcium-sensing R/CaSR Antibody (HL 1499) - Azide and BSA Free [NBP2-80607] - Detection of Calcium Sensing Receptor in transfected 293 cell lysate using NB 100-1830. ECL exposure, 5 minutes. In this photo, the protein has aggregated and thus the higher MW band as expected. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-HL-1499-Azide-and-BSA-Free-Western-Blot-NBP2-80607-img0002.jpg "Western Blot: Calcium-sensing R/CaSR Antibody (HL 1499) - Azide and BSA Free [NBP2-80607] - Detection of Calcium Sensing Receptor in transfected 293 cell lysate using NB 100-1830. ECL exposure, 5 minutes. In this photo, the protein has aggregated and thus the higher MW band as expected. Image from the standard format of this antibody.")
| Reactivity | Hu, Mu, Rt, RbSpecies Glossary |
| Applications | WB, Simple Western, ELISA, Flow, Func, ICC/IF, IHC, IP |
| Clone | HL 1499 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | N-terminal region of human Calcium Sensing Receptor. [UniProt# P41180] |
| Localization | Cytoplasmic |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CASR |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | In Western blot transfected samples were tested, where a band was seen ~130 kDa and a large smear at the top of the gel. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Theoretical MW | 130 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | Tris-Glycine, 0.15 M NaCl |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Calcium-sensing R/CaSR Antibody (NBP2-80607)Find related products by research area.
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| Gene Symbol | CASR |
![Immunocytochemistry/Immunofluorescence: Calcium-sensing R/CaSR Antibody (HL 1499) - Azide and BSA Free [NBP2-80607] - U2OS cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with antibody at 5 ug/mL overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-HL-1499-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80607-img0003.jpg "Immunocytochemistry/Immunofluorescence: Calcium-sensing R/CaSR Antibody (HL 1499) - Azide and BSA Free [NBP2-80607] - U2OS cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with antibody at 5 ug/mL overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.")
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (HL 1499) - Azide and BSA Free [NBP2-80607] - Analysis of FFPE human brain using Calcium-sensing R/CaSR antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxid](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-HL-1499-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80607-img0004.jpg "Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (HL 1499) - Azide and BSA Free [NBP2-80607] - Analysis of FFPE human brain using Calcium-sensing R/CaSR antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxid")
![Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499) - Azide and BSA Free [NBP2-80607] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using calcium sensing receptor antibody (dark blue). Isotype control shown in orange. Antibody at 1 ug/1 x 10^6 cells. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-HL-1499-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80607-img0007.jpg "Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499) - Azide and BSA Free [NBP2-80607] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using calcium sensing receptor antibody (dark blue). Isotype control shown in orange. Antibody at 1 ug/1 x 10^6 cells. Image from the standard format of this antibody.")
![Western Blot: Calcium-sensing R/CaSR Antibody (HL 1499) - Azide and BSA Free [NBP2-80607] - Analysis of Calcium Sensing Receptor expression in rat kidney tissue. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-HL-1499-Azide-and-BSA-Free-Western-Blot-NBP2-80607-img0001.jpg "Western Blot: Calcium-sensing R/CaSR Antibody (HL 1499) - Azide and BSA Free [NBP2-80607] - Analysis of Calcium Sensing Receptor expression in rat kidney tissue. Image from the standard format of this antibody.")
![Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499) - Azide and BSA Free [NBP2-80607] - An intracellular stain was performed on Hek293 cells with Calcium-sensing R/CaSR Antibody (HL 1499) NB100-1830PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-HL-1499-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80607-img0005.jpg "Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499) - Azide and BSA Free [NBP2-80607] - An intracellular stain was performed on Hek293 cells with Calcium-sensing R/CaSR Antibody (HL 1499) NB100-1830PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Phycoerythrin.")
![Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499) - Azide and BSA Free [NBP2-80607] - An intracellular stain was performed on HEK293 cells with Calcium-sensing R/CaSR Antibody [HL 1499] NB100-1830F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to FITC.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-HL-1499-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80607-img0006.jpg "Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499) - Azide and BSA Free [NBP2-80607] - An intracellular stain was performed on HEK293 cells with Calcium-sensing R/CaSR Antibody [HL 1499] NB100-1830F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to FITC.")
![Simple Western: Calcium-sensing R/CaSR Antibody (HL 1499) - Azide and BSA Free [NBP2-80607] - Image shows a specific band for Calcium Sensing Receptor in 0.5 mg/mL of HEK293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-HL-1499-Azide-and-BSA-Free-Simple-Western-NBP2-80607-img0008.jpg "Simple Western: Calcium-sensing R/CaSR Antibody (HL 1499) - Azide and BSA Free [NBP2-80607] - Image shows a specific band for Calcium Sensing Receptor in 0.5 mg/mL of HEK293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image from the standard format of this antibody.")
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