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Calcium-sensing R/CaSR Antibody (HL 1499) [FITC]

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Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499) [FITC] [NB100-1830F] - An intracellular stain was performed on Hek293 cells with Calcium-sensing R/CaSR Antibody [HL 1499] NB100-1830F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.
Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499) [FITC] [NB100-1830F] - An intracellular stain was performed on Hek293 cells with Calcium-sensing R/CaSR Antibody [HL 1499] NB100-1830F (blue) and a matched ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, RbSpecies Glossary
Applications WB, Simple Western, ELISA, Flow, Func, IP
Clone
HL 1499
Clonality
Monoclonal
Host
Mouse
Conjugate
FITC

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-1830AF350
Alexa Fluor 405
NB100-1830AF405
Alexa Fluor 488
NB100-1830AF488
Alexa Fluor 532
NB100-1830AF532
Alexa Fluor 594
NB100-1830AF594
Alexa Fluor 647
NB100-1830AF647
Alexa Fluor 700
NB100-1830AF700
Alexa Fluor 750
NB100-1830AF750
Biotin
NB100-1830B
DyLight 350
NB100-1830UV
DyLight 405
NB100-1830V
DyLight 488
NB100-1830G
DyLight 550
NB100-1830R
DyLight 594
NB100-1830DL594
DyLight 650
NB100-1830C
DyLight 680
NB100-1830FR
DyLight 755
NB100-1830IR
HRP
NB100-1830H
Janelia Fluor 549
NB100-1830JF549
Janelia Fluor 646
NB100-1830JF646
Unconjugated
NB100-1830
mFluor Violet 450 SE
NB100-1830MFV450
mFluor Violet 500 SE
NB100-1830MFV500
mFluor Violet 610 SE
NB100-1830MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB100-1830
BSA Free
NB100-1830
Azide and BSA Free
NBP2-80607
BSA Free
NB100-1830SS
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Calcium-sensing R/CaSR Antibody (HL 1499) [FITC] Summary

Immunogen
N-terminal region of human Calcium Sensing Receptor. [UniProt# P41180]
Localization
Cytoplasmic
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CASR
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry
  • Immunoprecipitation
  • Radioimmunoassay
  • Simple Western
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Publications
Read Publication using NB100-1830F.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Alternate Names for Calcium-sensing R/CaSR Antibody (HL 1499) [FITC]

  • calcium-sensing receptor
  • CAR
  • CaSR
  • EIG8
  • extracellular calcium-sensing receptor
  • FHH
  • FIH
  • GPRC2A
  • GPRC2AHHC
  • HHC1
  • hypocalciuric hypercalcemia 1
  • NSHPT
  • parathyroid Ca(2+)-sensing receptor 1
  • Parathyroid cell calcium-sensing receptor
  • PCaR1
  • PCAR1MGC138441

Background

Calcium Sensing Receptor (CaSR) was the first GPCR identified whose main physiological ligand is an ion and it has the ability to sense extracellular concentration of Ca2+. CaSR's activity is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system and it is capable of interaction with VCP, RNF19A (execute CASR's ubiquitination) and ARRB1. CaSR is expressed primarily in chief cells of parathyroid glands where, when activated by high Ca2+ concentrations, it decreases PTH release (calcium-retaining hormone) to maintain Ca2 +. Alternatively, under hypocalcemia conditions, CaSR is inactive and PTH is released which restores normocalcemia by acting on kidney as well as intestine to increase Ca2 + absorption, and on bone, to mobilise skeletal Ca2 +. CaSR is also expressed in other tissues which do not play an obvious role in Ca2 + homeostasis (breast, blood vessels, liver, and placenta) and altered CaSR expression and/or activity have been found associated with parathyroid glands disorders, osteoporosis, vascular calcification and cancer. CaSR is implicated in regulation of diverse processes that includes hormone secretion, gene expression, ion channel activity, modulation of inflammation, proliferation, differentiation, and apoptosis, depending on cell type, and therefore, represents a key molecule in physiology. Defects in CaSR have been linked to familial hypocalciuric hypercalcemia type 1 (FHH), neonatal severe primary hyperparathyroidism (NSHPT), familial isolated hypoparathyroidism (FIH) and epilepsy, idiopathic generalized type 8 (EIG8).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CASR