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Calbindin D-28K Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP1-86664] - Staining in human kidney and lymph node tissues using anti-CALB1 antibody. Corresponding CALB1 RNA-seq data are ...read more
Independent Antibodies: Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP1-86664] - Staining of human cerebellum, kidney, liver and lymph node using Anti-CALB1 antibody NBP1-86664 (A) shows similar ...read more
Western Blot: Calbindin D-28K Antibody [NBP1-86664] - Analysis in human kidney tissue.
Immunocytochemistry/ Immunofluorescence: Calbindin D-28K Antibody [NBP1-86664] - Staining of human cell line U-251 MG shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP1-86664] - Staining of human lymph node using Anti-CALB1 antibody NBP1-86664.
Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP1-86664] - Staining of human kidney shows high expression.
Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP1-86664] - Staining of human liver.
Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP1-86664] - Staining of human cerebellum.
Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP1-86664] - Staining of human lymp node.
Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP1-86664] - Staining of human kidney using Anti-CALB1 antibody NBP1-86664.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Calbindin D-28K Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: QSSLITASQFFEIWLHFDADGSGYLEGKELQNLIQELQQARKKAGLELSPEMKTFVDQYGQRDDGKIGIVELAHVLPTEENFLLLFRCQQ
Marker
Neuronal Marker
Predicted Species
Mouse (98%), Rat (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CALB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:5000 - 1:10000
  • Immunohistochemistry-Paraffin 1:5000 - 1:10000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Calbindin D-28K Protein (NBP1-86664PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Calbindin D-28K Antibody

  • CAB27
  • CALB
  • calbindin 1, (28kD)
  • calbindin 1, 28kDa
  • Calbindin D28
  • calbindin
  • D-28K
  • RTVL-H protein
  • Vitamin D-dependent calcium-binding protein, avian-type

Background

Calbindin D28k is a member of a large family of intracellular calcium-binding proteins, containing EF-hand calcium binding motifs, and related to calmodulin and troponin-C. The biological roles of Calbindin D28k include calcium regulation and calcium-dependent signalling in neurons and during development. Decreases in Calbindin D28k abundance, or loss of Calbindin D28k immunoreactivity, is found in models of neurological disorders such as epilepsy, and in neurodegenerative diseases. Calbindin antibody is often used as a marker for cerebellum Purkinje cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

FAQs for Calbindin D-28K Antibody (NBP1-86664). (Showing 1 - 1 of 1 FAQ).

  1. I am doing research on Neuroscience and I would like to use some antibodies, especially in Western Blots, but I could do other assays: - alpha7 nicotinic cholinergic receptor; - parvalbumin; - calbindin D28K; The preferred reactivity is anti-human and anti-mouse. Do you have these antibodies? Are they in stock? And what about the prizes: How much are they? How much are the shipping costs? Could you make me any offer?
    • This link will take you to all of our alpha7 nicotinic cholingergic receptor antibodies currently available for purchase. Please see the following link for our Calbinin D28K antibodies reactive against mouse and human. We currently do not have any Parvalbumin antibodies that have been tested in mouse. I would like to introduce you to our Innovators Reward Program. Try our primary antibodies in an untested species or application, without the financial risk of failure. The pricing and availability of our products depends on your country.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol CALB1