c-Myc Antibody [HRP]

The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is a multifunctional, nuclear phosphoprotein that functions as a transcription factor with a theoretical molecular weight of 62kDa. However, c-Myc is extremely labile and is degraded very quickly even in extracts prepared with boiling SDS sample buffer, such that a molecular weight of ~40 kDa has been observed. c-Myc is part of a heterodimeric complex with Max that acts as a potent transcriptional activator. c-Myc is modified by glycosylation and phosphorylation and has been shown to interact with numerous proteins including SMAD2, SMAD3, LSD1/KDM1A, MAD, and Sp1 (1).

A basic Helix-Loop-Helix, Leucine Zipper domain (bHLH/LZ), designated Max, specifically associates with c-Myc, N-Myc and L-Myc proteins. The Myc-Max complex binds to DNA in a sequence-specific manner under conditions where neither Max nor Myc exhibit appreciable binding. Max can also form heterodimers with other bHLH-Zip proteins, Mad and Mxi1. c-Myc plays a role in cell cycle progression, apoptosis, cellular transformation and angiogenesis (2). Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of cancers including B-cell Lymphomas, acute myeloid leukemia, glioblastoma, stomach adenocarcinoma, and prostate adenocarcinoma (3).

References

1. Wilkinson, D. S., Tsai, W. W., Schumacher, M. A., & Barton, M. C. (2008). Chromatin-bound p53 anchors activated Smads and the mSin3A corepressor to confer transforming-growth-factor-beta-mediated transcription repression. Mol Cell Biol, 28(6), 1988-1998. doi:10.1128/mcb.01442-07

2. Pedrosa, A. R., Bodrug, N., Gomez-Escudero, J., Carter, E. P., Reynolds, L. E., Georgiou, P. N., . . . Hodivala-Dilke, K. M. (2019). Tumor Angiogenesis Is Differentially Regulated by Phosphorylation of Endothelial Cell Focal Adhesion Kinase Tyrosines-397 and -861. Cancer Res, 79(17), 4371-4386. doi:10.1158/0008-5472.Can-18-3934

3. Nagasaka, M., Tsuzuki, K., Ozeki, Y., Tokugawa, M., Ohoka, N., Inoue, Y., & Hayashi, H. (2019). Lysine-Specific Demethylase 1 (LSD1/KDM1A) Is a Novel Target Gene of c-Myc. Biol Pharm Bull, 42(3), 481-488. doi:10.1248/bpb.b18-00892

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: B/N, ICC/IF, IP, S-ELISA, WB.

Showing Publications 1 - 10 of 10.

Publications using NB600-341	Applications	Species
BerY N, Cruz-Migoni A, Bataille CJ et al. BRET-based RAS biosensors that show a novel small molecule is an inhibitor of RAS-effector protein-protein interactions. Elife. Jul 10 2018 [PMID: 29989546] (WB, Human)	WB	Human
Moutel S, Bery N, Bernard V et al. NaLi-H1: A universal synthetic library of humanized nanobodies providing highly functional antibodies and intrabodies. Elife. 2016-07-19 [PMID: 27434673] (ICC/IF) Details: This citation used the HRP version of this antibody.	ICC/IF
Kuroiwa M, Hamada M, Hieda E et al. Muscarinic receptors acting at pre- and post-synaptic sites differentially regulate dopamine/DARPP-32 signaling in striatonigral and striatopallidal neuron Neuropharmacology 2012-12-01 [PMID: 22971543] (IP) Details: This citation used the HRP version of this antibody.	IP
Yancopoulous G, Papadopoulos N, Murphy A et al. ANTI-PCSK9 ANTIBODIES WITH pH-DEPENDENT BINDING CHARACTERISTICS World Intellectual Property Organization WO 2014/028354 Al 2014 Feb 20 (B/N, Human)	B/N	Human
Orengo J, Allinne, J ANTI-IL-25 ANTIBODIES AND USES THEREOF United States Patent Application 2016/0083466 A1 2016 Mar 24 (S-ELISA, Human)	S-ELISA	Human
Keller L Conception de nano-anticorps conformationnels comme nouveaux outils d'etude de l'activite des GTPases de la sous-famille RHOA Thesis 2017-01-20 (WB, Human)	WB	Human
Upchurch CM, Yeudall S, Pavelec CM Et al. Targeting oxidized phospholipids by AAV-based gene therapy in mice with established hepatic steatosis prevents progression to fibrosis Sci Adv 2022-07-20 [PMID: 35857497] (WB, Mouse) Details: Citation using the HRP version of this antibody.	WB	Mouse
Bery N, Keller L, SouliE M et al. A Targeted Protein Degradation Cell-Based Screening for Nanobodies Selective toward the Cellular RHOB GTP-Bound Conformation Cell Chem Biol. 2019-11-21 [PMID: 31522999] Details: Citation used the HRP format of this antibody.
Orengo J, Allinne J ANTI-IL-25 ANTIBODIES AND USES THEREOF United States Patent Application 2016/0083466 A1 2016 Mar 24 (S-ELISA, Human)	S-ELISA	Human
Bery N, Bataille CJR, Russell A et al. A cell-based screening method using an intracellular antibody for discovering small molecules targeting the translocation protein LMO2 Science advances Apr 1 2021 [PMID: 33837087] (WB, Human)	WB	Human

Images	Ratings	Applications	Species	Date	Details
	5 reviewed by: Stacy Ingher	ELISA	Human	06/15/2010	View Summary Application ELISA Sample Tested Private Species Human

Array NB600-341

• c-Myc Antibodies
• c-Myc ELISA Kits
• c-Myc Lysate
• c-Myc Proteins