![Western Blot: BTN1A1/Butyrophilin Antibody (OTI7B5) - Azide and BSA Free [NBP2-70299] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTN1A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTN1A1.](http://images.novusbio.com/fullsize/BTN1A1-Butyrophilin-Antibody-OTI7B5-Azide-and-BSA-Free-Western-Blot-NBP2-70299-img0002.jpg "Western Blot: BTN1A1/Butyrophilin Antibody (OTI7B5) - Azide and BSA Free [NBP2-70299] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTN1A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTN1A1.")
| Reactivity | Hu, CaSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | OTI7B5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Full length human recombinant protein of human BTN1A1 (NP_001723) produced in HEK293T cell. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | BTN1A1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 58.8 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
![Simple Western Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Simple_Western_21888.jpg)
![Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunocytochemistry__Immunofluorescence_17777.jpg)
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![Western Blot Myeloperoxidase/MPO Antibody [Unconjugated]](https://images.novusbio.com/images2/Myeloperoxidase_AF3667_Western_Blot_5321.jpg)
![Simple Western Myeloperoxidase/MPO Antibody [Unconjugated]](https://images.novusbio.com/images2/Myeloperoxidase_AF3667_Simple_Western_16637.jpg)
![Immunocytochemistry Myeloperoxidase/MPO Antibody [Unconjugated]](https://images.novusbio.com/images2/Myeloperoxidase_AF3667_Immunocytochemistry__Immunofluorescence_17390.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | BTN1A1 |
![Immunocytochemistry/Immunofluorescence: BTN1A1/Butyrophilin Antibody (OTI7B5) - Azide and BSA Free [NBP2-70299] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY BTN1A1.](http://images.novusbio.com/fullsize/BTN1A1-Butyrophilin-Antibody-OTI7B5-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70299-img0006.jpg "Immunocytochemistry/Immunofluorescence: BTN1A1/Butyrophilin Antibody (OTI7B5) - Azide and BSA Free [NBP2-70299] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY BTN1A1.")
![Immunohistochemistry: BTN1A1/Butyrophilin Antibody (OTI7B5) - Azide and BSA Free [NBP2-70299] - Staining of paraffin-embedded Human colon tissue using anti-BTN1A1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BTN1A1-Butyrophilin-Antibody-OTI7B5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70299-img0013.jpg "Immunohistochemistry: BTN1A1/Butyrophilin Antibody (OTI7B5) - Azide and BSA Free [NBP2-70299] - Staining of paraffin-embedded Human colon tissue using anti-BTN1A1 mouse monoclonal antibody.")
![Flow Cytometry: BTN1A1/Butyrophilin Antibody (OTI7B5) - Azide and BSA Free [NBP2-70299] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-BTN1A1 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/BTN1A1-Butyrophilin-Antibody-OTI7B5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70299-img0005.jpg "Flow Cytometry: BTN1A1/Butyrophilin Antibody (OTI7B5) - Azide and BSA Free [NBP2-70299] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-BTN1A1 antibody, and then analyzed by flow cytometry.")
![Western Blot: BTN1A1/Butyrophilin Antibody (OTI7B5) - Azide and BSA Free [NBP2-70299] - Analysis of extracts (35ug) from 9 different cell lines by using anti-BTN1A1 monoclonal antibody.](http://images.novusbio.com/fullsize/BTN1A1-Butyrophilin-Antibody-OTI7B5-Azide-and-BSA-Free-Western-Blot-NBP2-70299-img0001.jpg "Western Blot: BTN1A1/Butyrophilin Antibody (OTI7B5) - Azide and BSA Free [NBP2-70299] - Analysis of extracts (35ug) from 9 different cell lines by using anti-BTN1A1 monoclonal antibody.")
![Immunohistochemistry: BTN1A1/Butyrophilin Antibody (OTI7B5) - Azide and BSA Free [NBP2-70299] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-BTN1A1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BTN1A1-Butyrophilin-Antibody-OTI7B5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70299-img0007.jpg "Immunohistochemistry: BTN1A1/Butyrophilin Antibody (OTI7B5) - Azide and BSA Free [NBP2-70299] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-BTN1A1 mouse monoclonal antibody.")
![Immunohistochemistry: BTN1A1/Butyrophilin Antibody (OTI7B5) - Azide and BSA Free [NBP2-70299] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-BTN1A1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BTN1A1-Butyrophilin-Antibody-OTI7B5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70299-img0008.jpg "Immunohistochemistry: BTN1A1/Butyrophilin Antibody (OTI7B5) - Azide and BSA Free [NBP2-70299] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-BTN1A1 mouse monoclonal antibody.")
![Immunohistochemistry: BTN1A1/Butyrophilin Antibody (OTI7B5) - Azide and BSA Free [NBP2-70299] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-BTN1A1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BTN1A1-Butyrophilin-Antibody-OTI7B5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70299-img0009.jpg "Immunohistochemistry: BTN1A1/Butyrophilin Antibody (OTI7B5) - Azide and BSA Free [NBP2-70299] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-BTN1A1 mouse monoclonal antibody.")
![Immunohistochemistry: BTN1A1/Butyrophilin Antibody (OTI7B5) - Azide and BSA Free [NBP2-70299] - Staining of paraffin-embedded Human pancreas tissue using anti-BTN1A1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BTN1A1-Butyrophilin-Antibody-OTI7B5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70299-img0010.jpg "Immunohistochemistry: BTN1A1/Butyrophilin Antibody (OTI7B5) - Azide and BSA Free [NBP2-70299] - Staining of paraffin-embedded Human pancreas tissue using anti-BTN1A1 mouse monoclonal antibody.")
![Immunohistochemistry: BTN1A1/Butyrophilin Antibody (OTI7B5) - Azide and BSA Free [NBP2-70299] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-BTN1A1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BTN1A1-Butyrophilin-Antibody-OTI7B5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70299-img0011.jpg "Immunohistochemistry: BTN1A1/Butyrophilin Antibody (OTI7B5) - Azide and BSA Free [NBP2-70299] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-BTN1A1 mouse monoclonal antibody.")
![Immunohistochemistry: BTN1A1/Butyrophilin Antibody (OTI7B5) - Azide and BSA Free [NBP2-70299] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-BTN1A1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BTN1A1-Butyrophilin-Antibody-OTI7B5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70299-img0012.jpg "Immunohistochemistry: BTN1A1/Butyrophilin Antibody (OTI7B5) - Azide and BSA Free [NBP2-70299] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-BTN1A1 mouse monoclonal antibody.")
![Immunohistochemistry: BTN1A1/Butyrophilin Antibody (OTI7B5) - Azide and BSA Free [NBP2-70299] - Staining of paraffin-embedded Human thyroid tissue using anti-BTN1A1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BTN1A1-Butyrophilin-Antibody-OTI7B5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70299-img0014.jpg "Immunohistochemistry: BTN1A1/Butyrophilin Antibody (OTI7B5) - Azide and BSA Free [NBP2-70299] - Staining of paraffin-embedded Human thyroid tissue using anti-BTN1A1 mouse monoclonal antibody.")
![Flow Cytometry: BTN1A1/Butyrophilin Antibody (OTI7B5) - Azide and BSA Free [NBP2-70299] - Analysis of Hela cells, using anti-BTN1A1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/BTN1A1-Butyrophilin-Antibody-OTI7B5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70299-img0003.jpg "Flow Cytometry: BTN1A1/Butyrophilin Antibody (OTI7B5) - Azide and BSA Free [NBP2-70299] - Analysis of Hela cells, using anti-BTN1A1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
![Flow Cytometry: BTN1A1/Butyrophilin Antibody (OTI7B5) - Azide and BSA Free [NBP2-70299] - Analysis of Jurkat cells, using anti-BTN1A1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/BTN1A1-Butyrophilin-Antibody-OTI7B5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70299-img0004.jpg "Flow Cytometry: BTN1A1/Butyrophilin Antibody (OTI7B5) - Azide and BSA Free [NBP2-70299] - Analysis of Jurkat cells, using anti-BTN1A1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
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![N/A Kallikrein 3/PSA [HRP]](https://images.novusbio.com/images2/Kallikrein_3_DKK300_ELISA_124.jpg)
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![N/A SHBG [HRP]](https://images.novusbio.com/images2/SHBG_DSHBG0_ELISA_1106.jpg)
![N/A SHBG [HRP]](https://images.novusbio.com/images2/SHBG_DSHBG0_ELISA_1107.jpg)
![N/A SHBG [HRP]](https://images.novusbio.com/images2/SHBG_DSHBG0_ELISA_2581.jpg)
![Western Blot GATA-3 Antibody [Unconjugated]](https://images.novusbio.com/images2/GATA-3_AF2605_Western_Blot_5199.jpg)
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