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Brachyury Antibody

Images

 
Western Blot: Brachyury Antibody [NBP2-24676] - Analysis of Brachyury in U87 cell lysate. Lane 1 shows pre-immune sera, lane 2 shows this antibody tested at 2 ug/mL.
Immunohistochemistry-Paraffin: Brachyury Antibody [NBP2-24676] - Analysis of Brachyury in FFPE human brain tissue using an isotype control (top left) and this antibody (bottom left, right) at 5 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, Pm, Rt, Ca, PmSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

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Brachyury Antibody Summary

Immunogen
A portion of amino acids 350-400 of human Brachyury was used as the immunogen.
Marker
Mesoderm Marker
Predicted Species
Rat (93%), Canine (93%), Monkey (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TBXT
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:20 - 1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Western Blot 1 - 3 ug/mL
Publications
Read Publications using
NBP2-24676 in the following applications:

  • 5 publications
  • WB
    3 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28132888).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for Brachyury Antibody

  • brachyury protein
  • Brachyury
  • MGC104817
  • Protein T
  • SAVA
  • T brachyury (mouse) homolog
  • T brachyury homolog
  • T Brachyury Transcription Factor
  • T, brachyury homolog (mouse)
  • T-Box Transcription Factor T
  • TBXT
  • TF
  • TFT

Background

Brachyury / Bry is encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Brachyury Antibody (NBP2-24676)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(5)
WB
(3)
All Applications
Filter By Species
Human
(5)
Mouse
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using NBP2-24676 Applications Species
ChadlY DM, Oleksijew AM, Coots kS et al. Full Factorial Microfluidic Designs and Devices for Parallelizing Human Pluripotent Stem Cell Differentiation. SLAS Technol. 2018-07-01 [PMID: 29995450] (ICC/IF, Human) ICC/IF Human
Qu Y, Zhou B, Yang W et al. Transcriptome and proteome characterization of surface ectoderm cells differentiated from human iPSCs. Sci Rep. 2016-08-23 [PMID: 27550649] (ICC/IF, WB, Human) ICC/IF, WB Human
Yukawa H, Noguchi H, Hayashi S. Embryonic body formation using the tapered soft stencil for cluster culture device. Biomaterials. 2011-05-01 [PMID: 21354615] (ICC/IF)

Details:
Product cited: Brachyury (IMG-6533A). IF/ICC: Induced pluripotent stem (iPS) cells, Fig 1 & 2.
ICC/IF
Qu Y, Cui X, Sareen D et al. In vitro induction of mammary-like differentiation from human pluripotent stem cells Patent 2018-08-02 (WB, Human) WB Human
Song MH, Choi SC, Noh JM et al. LEFTY-PITX2 signaling pathway is critical for generation of mature and ventricular cardiac organoids in human pluripotent stem cell-derived cardiac mesoderm cells Biomaterials 2021-09-21 [PMID: 34571434] (ICC/IF, Human) ICC/IF Human
Indana D, Agarwal P, Bhutani N, Chaudhuri O Viscoelasticity and Adhesion Signaling in Biomaterials Control Human Pluripotent Stem Cell Morphogenesis in 3D Culture Advanced materials (Deerfield Beach, Fla.) 2021-09-09 [PMID: 34499389] (ICC/IF) ICC/IF
Qu Y, Han B, Gao B et al. Differentiation of Human Induced Pluripotent Stem Cells to Mammarylike Organoids Stem Cell Reports. 2017-02-14 [PMID: 28132888] (WB, Mouse, Human) WB Mouse, Human

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Brachyury Antibody (NBP2-24676) (0)

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Secondary Antibodies

 

Isotype Controls

Additional Brachyury Products

Research Areas for Brachyury Antibody (NBP2-24676)

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Blogs on Brachyury

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Bioinformatics

Gene Symbol TBXT
Uniprot