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Brachyury Antibody (6C12) - BSA Free

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Western Blot: Brachyury Antibody (6C12) [NBP2-37473] - Analysis using T mAb against HEK293 (1) and T (AA: 218-352)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot: Brachyury Antibody (6C12) [NBP2-37473] - Analysis using T mAb against HEK293 (1) and T (AA: 218-352)-hIgGFc transfected HEK293 (2) cell lysate.
Flow Cytometry: Brachyury Antibody (6C12) [NBP2-37473] - Analysis of A549 cells using T mouse mAb (green) and negative control (red).
Western Blot: Brachyury Antibody (6C12) [NBP2-37473] - Analysis using T mAb against human T recombinant protein. (Expected MW is 40.3 kDa)
ELISA: Brachyury Antibody (6C12) [NBP2-37473] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, CyTOF-ready
Clone
6C12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP2-37473-0.025ml
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Brachyury Antibody (6C12) - BSA Free Summary

Immunogen
Purified recombinant fragment of human Brachyury (AA: 218-352) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TBXT
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Western Blot 1:500 - 1:2000
Application Notes
This antibody is Cytof ready.
Theoretical MW
47.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Brachyury Antibody (6C12) - BSA Free

  • brachyury protein
  • Brachyury
  • MGC104817
  • Protein T
  • SAVA
  • T brachyury (mouse) homolog
  • T brachyury homolog
  • T Brachyury Transcription Factor
  • T, brachyury homolog (mouse)
  • T-Box Transcription Factor T
  • TBXT
  • TF
  • TFT

Background

Brachyury / Bry is encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol TBXT