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Human beta-NGF ELISA Kit (Chemiluminescence)

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ELISA: Human beta-NGF ELISA Kit (Chemiluminescence) [NBP2-62775] - Samples were spiked with high concentrations of Human beta-NGF and diluted with Reference Standard & Sample Diluent to produce samples with values ...read more
ELISA: Human beta-NGF ELISA Kit (Chemiluminescence) [NBP2-62775] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
7.81-500 pg/mL
Sensitivity
4.69 pg/mL

Order Details

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Human beta-NGF ELISA Kit (Chemiluminescence) Summary

Specificity
This kit recognizes Human NGF in samples. No significant cross-reactivity or interference between Human NGF and analogues was observed.
Standard Curve Range
7.81-500 pg/mL
Sensitivity
4.69 pg/mL
Assay Type
Sandwich-CLIA
Inter-Assay
CV% < 8.85%
Intra-Assay
CV% < 8.85%
Spike Recovery
97-113%
Sample Volume
100 uL
Kit Type
ELISA Kit (Chemiluminescence)
Gene
NGF

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro CLIA Plate
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Substrate Reagent A
  12. Substrate Reagent B

Alternate Names for Human beta-NGF ELISA Kit (Chemiluminescence)

  • Beta nerve growth factor precursor
  • beta-nerve growth factor
  • betaNGF
  • beta-NGF
  • HSAN5
  • MGC161426
  • nerve growth factor (beta polypeptide)
  • nerve growth factor, beta subunit
  • NGFB
  • NGFBMGC161428

Background

Nerve growth factor (NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and some sensory neurons, and is composed by 3 types of subunits (alpha, beta and gamma) that specifically interact to form a 7S, 130 kDa molecular weight complex. The beta-NGF subunit is a secreted protein that forms a homodimer before being incorporated into the NGF complex. The human and mouse sequences are very similar and mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5). Deregulation of its expression has been associated with allergic rhinitis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol NGF