beta Amyloid [Pyro Glu3] Antibody

Beta amyloid, also known as Abeta, is a peptide that varies in length from 39 to 43 amino acids and is processed from the transmembrane protein amyloid precursor protein (APP) by beta-secretase (BACE-1) and gamma-secretase. Beta amyloid [1-40], beta amyloid [1-42], and beta amyloid [1-43] peptides result from cleavage of APP after residues 40, 42, and 43, respectively. The aggregation of beta amyloid monomers produces insoluble oligomers and protofibrils. The longer "stickier" forms of beta amyloid, particularly ABeta42, are known as the main component of amyloid plaques, which are deposits (senile plaques or neuritic plaques) found in the brains of patients with Alzheimer's disease (AD) (1,2). Research indicates that intraneuronal beta amyloid accumulation may be an important proximal neurotoxic event in the pathogenesis of Alzheimer's disease. Abeta deposits have also been reported in the hearts of AD patients (3).

Pyroglutamate amyloid beta peptides (pGlu-Abeta) are N-terminal truncations in which the N-terminal glutamate is cyclized to pyroglutamate resulting in pGlu-Abeta (3-40/42 and 11-40/42) (4). This pyrE modification may have a greater propensity to aggregate under physiological conditions and has been implicated as the molecular species responsible for seeding larger oligomers of amyloid beta.

References

1. Chen GF, Xu TH, Yan Y, Zhou YR, Jiang Y, Melcher K, Xu HE. (2017) Amyloid beta: structure, biology and structure-based therapeutic development. Acta Pharmacol Sin. 38(9):1205-1235. PMID: 28713158

2. De-Paula VJ1, Radanovic M, Diniz BS, Forlenza OV. (2012) Alzheimer's disease. Subcell Biochem. 65:329-52. PMID: 23225010

3. Schaich CL, Maurer MS, Nadkarni NK. (2019) Amyloidosis of the Brain and Heart: Two Sides of the Same Coin? JACC Heart Fail. 7(2):129-131. PMID: 30704604

4. He W, Barrow CJ. (1999) The A beta 3-pyroglutamyl and 11-pyroglutamyl peptides found in senile plaque have greater beta-sheet forming and aggregation propensities in vitro than full-length A beta. Biochemistry. 38(33):10871-7. PMID: 10451383

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Mouse, Canine, Drosophila.

We have publications tested in 4 applications: IA, IF/IHC, IHC-Fr, IHC-FrFl.

Showing Publications 1 - 7 of 7.

Publications using NBP1-44048	Applications	Species
William J. Behof, Justin R. Haynes, Clayton A. Whitmore, Yiu-Yin Cheung, Mohammed N. Tantawy, Todd E. Peterson, Printha Wijesinghe, Joanne A. Matsubara, Wellington Pham Synthesis and Evaluation of a Novel PET Radioligand for Imaging Glutaminyl Cyclase Activity as a Biomarker for Detecting Alzheimer’s Disease ACS Sensors 2024-05-08 [PMID: 38718161]
Tan Z, GarduNo BM, Aburto PF et al. Cognitively impaired aged Octodon degus recapitulate major neuropathological features of sporadic Alzheimer's disease Acta neuropathologica communications 2022-12-19 [PMID: 36529803] (IHC-FrFl, Mouse) Details: Dilution used in IHC-FrFl 1:500	IHC-FrFl	Mouse
Spangenberg E, Severson PL, Hohsfield LA et al. Sustained microglial depletion with CSF1R inhibitor impairs parenchymal plaque development in an Alzheimer's disease model Nat Commun 2019-08-21 [PMID: 31434879] (IF/IHC, Mouse)	IF/IHC	Mouse
Davis PR, Giannini G, Rudolph K et al. AB vaccination in combination with behavioral enrichment in aged beagles: effects on cognition, AB, and microhemorrhages. Neurobiol Aging. [PMID: 27776266] (IHC-Fr, Canine)	IHC-Fr	Canine
Jonson M, Pokrzywa M, Starkenberg A et al. Systematic Ab Analysis in Drosophila Reveals High Toxicity for the 1-42, 3-42 and 11-42 Peptides, and Emphasizes N- and C-Terminal Residues PLoS ONE. 2015-07-25 [PMID: 26208119] (IA, Drosophila)	IA	Drosophila
Schilling S, Lauber T, Schaupp M, Manhart S, Scheel E, Bohm G, Demuth HU. On the seeding and oligomerization of pGlu-amyloid peptides (in vitro). Biochemistry;45(41):12393-9. 2006-10-17 [PMID: 17029395]
He W, Barrow CJ. The A beta 3-pyroglutamyl and 11-pyroglutamyl peptides found in senile plaque have greater beta-sheet forming and aggregation propensities in vitro than full-length A beta. Biochemistry;38(33):10871-7. 1999-08-17 [PMID: 10451383]

Array NBP1-44048

• beta Amyloid Antibodies
• beta Amyloid ELISAs