![ELISA: Human beta-Actin - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31944] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-31944_human-beta-actin-ready-to-use-elisa-kit-colorimetric-20620241533911.png "ELISA: Human beta-Actin - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31944] - Standard Curve Reference")
| Reactivity | HuSpecies Glossary |
| Applications | ELISA |
| Suitable Sample Type | Tissue homogenates, cell lysates, cell culture supernatants and other biological fluids |
| Standard Curve Range | 1.56 - 100 ng/mL (example only; lot dependent) |
| Sensitivity | 0.63 ng/mL (example only; lot dependent) |
| Description | The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time. Assay Length: 3 hours |
| Standard Curve Range | 1.56 - 100 ng/mL (example only; lot dependent) |
| Sensitivity | 0.63 ng/mL (example only; lot dependent) |
| Assay Type | Sandwich ELISA |
| Inter-Assay | %CV < 12 (example only; lot dependent) |
| Intra-Assay | %CV < 10 (example only; lot dependent) |
| Sample Volume | 100 uL |
| Kit Type | ELISA Kit (Colorimetric) |
| Gene | ACTB |
| Storage | Storage of components varies. See protocol for specific instructions. |
| Components |
|
Research Areas for beta-Actin ELISA Kit (NBP3-31944)Find related products by research area.
|
|
Application Focus: I see an increase in LC3, now what? By Christina Towers, PhD. Autophagy is highly conserved and tightly regulated process that all cell types use to recycle nutrients, particularly in the instance of stress1. As a result, even sm... Read full blog post. |
|
The use of Beta Actin (AC-15) as a loading control across multiple species Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs. Actin has six highly conserved isoforms, however beta and gamma actin are the two... Read full blog post. |
|
Tips on choosing an ideal loading control antibody for Western Blotting Western blotting is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. When p... Read full blog post. |
|
Beta-Actin's Role in Neuronal Plasticity Beta-Actin is a highly conserved protein involved in cell growth, cytoskeletal and extracellular support structures and cell migration. Because beta-Actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading contro... Read full blog post. |
|
ACTB - an abundant cytoskeletal component with applications for gene expression analysis Actin is the widely studied and ubiquitous cytoskeletal protein capable of forming dynamic microfilament structures. These filaments are essential for diverse cellular functions including cell shape, migration, cytokinesis, and intracellular traffi... Read full blog post. |
|
A New Standard in Antibody Testing - Simple Western Certified Antibodies The Western blot is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. The tradi... Read full blog post. |
| Gene Symbol | ACTB |
![Simple Western 14-3-3 zeta Antibody (818515) [Unconjugated]](https://images.novusbio.com/images2/1433_zeta_MAB2669_Simple_Western_16526.jpg)
![Western Blot 14-3-3 zeta Antibody (818515) [Unconjugated]](https://images.novusbio.com/images2/14-3-3_zeta_MAB2669_Western_Blot_12700.jpg)
![Immunohistochemistry 14-3-3 zeta Antibody (818515) [Unconjugated]](https://images.novusbio.com/images2/14-3-3_zeta_MAB2669_Immunohistochemistry_12730.jpg)
![Ubiquitin [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Ubiquitin [Unconjugated]")
![Flow Cytometry: beta-Actin Antibody [NB600-503] - An intracellular stain was performed on HeLa cells with beta-actin Antibody NB600-503AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](https://images.novusbio.com/images/beta-Actin-Antibody-Flow-Cytometry-NB600-503-img0013.jpg "Flow Cytometry: beta-Actin Antibody [NB600-503] - An intracellular stain was performed on HeLa cells with beta-actin Antibody NB600-503AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.")