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beta 2-Microglobulin Antibody (3G5H8) - BSA Free

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Western Blot: beta 2-Microglobulin Antibody (3G5H8) [NBP2-37287] - Western blot analysis using B2M mouse mAb against Hela (1), HEK293 (2), HepG2 (3),RAJI (4), A431 (5) and Jurkat (6) cell lysate.
Western Blot: beta 2-Microglobulin Antibody (3G5H8) [NBP2-37287] - Western blot analysis using B2M mouse mAb against Hela (1), HEK293 (2), HepG2 (3),RAJI (4), A431 (5) and Jurkat (6) cell lysate.
Immunohistochemistry: beta 2-Microglobulin Antibody (3G5H8) [NBP2-37287] - Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using B2M mouse mAb with DAB staining.
Flow Cytometry: beta 2-Microglobulin Antibody (3G5H8) [NBP2-37287] - Flow cytometric analysis of A431 cells using B2M mouse mAb (green) and negative control (red).
Western Blot: beta 2-Microglobulin Antibody (3G5H8) [NBP2-37287] - Western blot analysis using B2M mAb against human B2M (AA: 21-100) recombinant protein. (Expected MW is 35.4 kDa)
Western Blot: beta 2-Microglobulin Antibody (3G5H8) [NBP2-37287] - Western blot analysis using B2M mAb against HEK293 (1) and B2M (AA: 21-100)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry: beta 2-Microglobulin Antibody (3G5H8) [NBP2-37287] - Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using B2M mouse mAb with DAB staining.
ELISA: beta 2-Microglobulin Antibody (3G5H8) [NBP2-37287] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IHC, CyTOF-ready
Clone
3G5H8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP2-37287-0.025ml
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beta 2-Microglobulin Antibody (3G5H8) - BSA Free Summary

Immunogen
Purified recombinant fragment of human beta 2-Microglobulin (AA: 21-100) expressed in E. Coli.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
B2M
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
Application Notes
This antibody is Cytof ready.
Theoretical MW
13.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for beta 2-Microglobulin Antibody (3G5H8) - BSA Free

  • B2M
  • beta 2Microglobulin
  • beta 2-Microglobulin
  • beta chain of MHC class I molecules
  • beta-2-microglobin
  • beta-2-microglobulin

Background

Beta-2-microglobulin is a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells (Gussow et al., 1987 [PubMed 3312414]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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beta
2-Microglobulin Antib ...
NBP2-37287
beta 2-Microglobulin Antibody (3G5H8) - BSA Free
Species: Hu
Applications: WB, ELISA, Flow, IHC, CyTOF-ready

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Bioinformatics

Gene Symbol B2M