Home » BCL-W/BCL2L2 » BCL-W Antibodies » BCL-W/BCL2L2 Antibody [Unconjugated]
Novus Biologicals products are now on bio-techne.com

BCL-W/BCL2L2 Antibody [Unconjugated]

 (2 Publications)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Western blot shows lysates of WS-1 human fetal skin fibroblast cell line and CTLL-2 mouse cytotoxic T cell line. PVDF membrane was probed with 1.2 µg/mL Rabbit Anti-Human/Mouse Bcl-w Antigen Affinity-purified Polyclonal Antibody (Catalog # AF824) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). For additional reference, Recombinant Human Bcl-w Minus C-Terminus (Catalog # 824-BW) (1 ng) was included. A specific band for Bcl-w was detected at approximately 19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
Western blot shows lysates of WS-1 human fetal skin fibroblast cell line and CTLL-2 mouse cytotoxic T cell line. PVDF membrane was probed with 1.2 µg/mL Rabbit Anti-Human/Mouse Bcl-w Antigen Affinity-purified ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF824-SP
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

BCL-W/BCL2L2 Antibody [Unconjugated] Summary

Immunogen
KLH-coupled human/mouse Bcl-w synthetic peptide
Gly32-Asp51
Accession # Q92843
Specificity
Detects human and mouse Bcl-w in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BCL2L2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1.2 ug/mL
Publications
Read Publications using
AF824 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BCL-W/BCL2L2 Antibody [Unconjugated]

  • Apoptosis regulator Bcl-W
  • BCL2L2
  • Bcl2-L-2
  • BCL2-like 2
  • Bclw
  • Bcl-w
  • BCLWbcl-2-like protein 2
  • KIAA0271BCL2-L-2

Background

Bcl-w, also called Bcl-2 like protein 2, is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability (1, 2). Bcl-w is an anti-apoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. Bcl-w is required for normal sperm maturation (3, 4, 5). Natural Bcl-w contains a carboxyl-terminal mitochondria targeting sequence. Recombinant Bcl-w missing the mitochondrial targeting sequence maintains its ability to neutralize pro-apoptotic Bcl-2 family members. Neutralization by Bcl-w appears to be through binding the BH3 region of pro-apoptotic Bcl-2 family members. This activity does not require the mitochondrial targeting sequence.

  1. Gross, A. et al. (1999) Genes and Develop. 13:1899.
  2. Kroemer, G. (1997) Nature Med. 3:614.
  3. Ross, J.A., et al. (1998) Nat. Genet. 18:251.
  4. Print, C.G. et al. (1998) Proc. Natl. Acad. Sci. USA 95:12323.
  5. Yan, W. et al. (2000) Mol. Endocrin. 14:682.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Bcl-xL Antibody
NB100-56104
Western Blot Bcl-xL Antibody
Immunohistochemistry-Paraffin Bcl-xL Antibody
Western Blot Bcl-xL Antibody
(1 Review)
(3 Publications)
Species: Ca, Hu, Mu, Rt
Applications: IHC, IHC-Fr, IHC-P, IP, WB
Bcl-2 Antibody
NB100-56098
Western Blot Bcl-2 Antibody
Western Blot Bcl-2 Antibody
Western Blot Bcl-2 Antibody
(2 Reviews)
(26 Publications)
Species: Ca, Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
Bax Antibody (6A7)
NBP1-28566
Western Blot Bax Antibody (6A7)
Western Blot Bax Antibody (6A7)
Immunohistochemistry-Frozen Bax Antibody (6A7)
(3 Reviews)
(21 Publications)
Species: Bv, Hu, Pm, Mu, Po, Rt
Applications: Func, IHC, IHC-Fr, IHC-P, WB
Mcl-1 Antibody
NB100-56146
Western Blot Mcl-1 Antibody
Immunohistochemistry-Paraffin Mcl-1 Antibody
(1 Review)
(5 Publications)
Species: Hu
Applications: IHC, IHC-P, IP, WB
BAK Antibody
[Unconjugated]
AF816
Western Blot BAK Antibody [Unconjugated]
Immunocytochemistry BAK Antibody [Unconjugated]
Immunohistochemistry BAK Antibody [Unconjugated]
(5 Publications)
Species: Hu
Applications: ICC, IHC, WB
Bim Antibody - BSA
Free
NBP1-76963
Western Blot Bim Antibody - BSA Free
Western Blot Bim Antibody - BSA Free
Western Blot Bim Antibody - BSA Free
(8 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, Simple Western, WB
BID Antibody
[Unconjugated]
AF860
Simple Western BID Antibody [Unconjugated]
Western Blot BID Antibody [Unconjugated]
Western Blot BID Antibody [Unconjugated]
(1 Review)
(40 Publications)
Species: Hu, Mu
Applications: IP, Simple Western, WB
Caspase-3 Antibody
[Unconjugated] - Acti ...
AF835
Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active
Immunohistochemistry Caspase-3 Antibody [Unconjugated] - Active
Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active
(7 Reviews)
(389 Publications)
Species: Hu, Mu
Applications: ICC, IHC
ATP6V0A1 Antibody
NBP1-89342
Immunocytochemistry/ Immunofluorescence ATP6V0A1 Antibody
Immunohistochemistry-Paraffin ATP6V0A1 Antibody
Immunohistochemistry-Paraffin ATP6V0A1 Antibody
(1 Review)
(15 Publications)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, IP, WB
RFC2 Antibody
(OTI2B2)
NBP2-45570
Flow Cytometry RFC2 Antibody (OTI2B2)
Immunohistochemistry RFC2 Antibody (OTI2B2)
Immunohistochemistry RFC2 Antibody (OTI2B2)
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, WB
RFC1 Antibody
[Unconjugated]
AF6457
Western Blot RFC1 Antibody [Unconjugated]
Species: Hu
Applications: WB
RFC4 Antibody
(OTI1A8)
NBP2-45946
Immunocytochemistry/ Immunofluorescence RFC4 Antibody (OTI1A8)
Immunohistochemistry RFC4 Antibody (OTI1A8)
Immunohistochemistry RFC4 Antibody (OTI1A8)
Species: Ca, Hu, Pm, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Bad Antibody
NB100-56080
Western Blot Bad Antibody
Immunocytochemistry/ Immunofluorescence Bad Antibody
Immunohistochemistry-Paraffin Bad Antibody
(1 Review)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, IP, WB
Noxa Antibody
(114C307.1) - Non-Rec ...
NB600-1159
Immunocytochemistry/ Immunofluorescence Noxa Antibody (114C307.1) - Non-Recombinant Monoclonal
Flow Cytometry Noxa Antibody (114C307.1) - Non-Recombinant Monoclonal
Flow Cytometry Noxa Antibody (114C307.1) - Non-Recombinant Monoclonal
(2 Reviews)
(77 Publications)
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
Cytochrome c
Antibody (7H8.2C12) - ...
NB100-56503
Flow Cytometry Cytochrome c Antibody (7H8.2C12) - BSA Free
Flow (Intracellular) Cytochrome c Antibody (7H8.2C12) - BSA Free
Western Blot Cytochrome c Antibody (7H8.2C12) - BSA Free
(61 Publications)
Species: Dr, Hu, Mu, Rb, Rt
Applications: Flow-CS, Flow-IC, Flow, IB, ICC/IF, IHC, IHC-P, Simple Western, WB
p53 Antibody (PAb
240) - BSA Free
NB200-103
Flow Cytometry p53 Antibody (PAb 240) - BSA Free
Flow Cytometry p53 Antibody (PAb 240) - BSA Free
Flow (Intracellular) p53 Antibody (PAb 240) - BSA Free
(3 Reviews)
(43 Publications)
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(820 Publications)
Species: Hu
Applications: BA
BCL-W/BCL2L2
Antibody [Unconjugate
AF824
BCL-W/BCL2L2 Antibody [Unconjugated]
Species: Hu, Mu
Applications: WB

Publications for BCL-W/BCL2L2 Antibody (AF824)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: Western Blot.


Filter By Application
Western Blot
(2)
All Applications
Filter By Species
Human
(1)
Mouse
(1)
All Species

Reviews for BCL-W/BCL2L2 Antibody (AF824) (0)

There are no reviews for BCL-W/BCL2L2 Antibody (AF824). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for BCL-W/BCL2L2 Antibody (AF824) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional BCL-W/BCL2L2 Products

Blogs on BCL-W/BCL2L2

There are no specific blogs for BCL-W/BCL2L2, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

HepG2 human hepatocellular carcinoma cell line was stained with Rabbit Anti-Human Klotho beta Monoclonal Antibody (Catalog # MAB58891, filled histogram) or Rabbit IgG Isotype Control Antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins.
Rabbit IgG Isotype Control [Unconjugated]
AB-105-C
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our BCL-W/BCL2L2 Antibody [Unconjugated] and receive a gift card or discount.


Bioinformatics

Gene Symbol BCL2L2
Uniprot