![Western Blot: Bcl 7A Antibody [NB100-769] - Staining of Human Lymphnode lysate (RIPA buffer, 35 ug total protein per lane). Antibody at 1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Bcl-7A-Antibody-Western-Blot-NB100-769-img0003.jpg "Western Blot: Bcl 7A Antibody [NB100-769] - Staining of Human Lymphnode lysate (RIPA buffer, 35 ug total protein per lane). Antibody at 1 ug/mL. Detected by chemiluminescence.")
| Reactivity | Hu, Mu, Rt, Po, Bv, CaSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-MKLEASQQNSEEM corresponding to C-Terminus according to NP_066273.1, NP_001019979.1. |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_066273.1; NP_001019979.1). |
| Predicted Species | Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | BCL7A |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Application Notes | WB: Approx. 26 kDa band observed in human lymph node lysates (calculated MW of 25.0 kDa band according to NP_066273.1). |
|
| Publications |
|
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: Bcl 7A Antibody [NB100-769] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Bcl-7A-Antibody-Immunocytochemistry-Immunofluorescence-NB100-769-img0004.jpg "Immunocytochemistry/Immunofluorescence: Bcl 7A Antibody [NB100-769] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).")
![Flow Cytometry: Bcl 7A Antibody [NB100-769] - Flow cytometric analysis of paraformaldehyde fixed Daudi cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Bcl-7A-Antibody-Flow-Cytometry-NB100-769-img0005.jpg "Flow Cytometry: Bcl 7A Antibody [NB100-769] - Flow cytometric analysis of paraformaldehyde fixed Daudi cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.")
![Western Blot Brg1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Brg1_AF5738_Western_Blot_5829.jpg)
![Immunocytochemistry Brg1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Brg1_AF5738_Immunocytochemistry_11663.jpg)
![Immunocytochemistry Brg1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Brg1_AF5738_Immunocytochemistry_11664.jpg)
