![Western Blot: Bcl-6 Antibody (BCL6/1475) - Azide and BSA Free [NBP2-59597] - Western Blot Analysis (A) Recombinant Protein (B) HepG2 cell lysate Bcl-6 Antibody (BCL6/1475).](http://images.novusbio.com/fullsize/Bcl-6-Antibody-BCL6-1475-Azide-and-BSA-Free-Western-Blot-NBP2-59597-img0003.jpg "Western Blot: Bcl-6 Antibody (BCL6/1475) - Azide and BSA Free [NBP2-59597] - Western Blot Analysis (A) Recombinant Protein (B) HepG2 cell lysate Bcl-6 Antibody (BCL6/1475).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, MA, CyTOF-ready |
| Clone | BCL6/1475 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | Positive Control:Raji or Ramos cells. Tonsil 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-59596). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous. |
| Immunogen | Recombinant human bcl-6 protein fragment (around aa256-389) (exact sequence is proprietary) (Uniprot: P41182) |
| Localization | Nuclear |
| Marker | Follicular Lymphoma Marker |
| Specificity | The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. Recognizes a protein of 95kDa, which is identified as Bcl-6. Antibody to bcl-6 is helpful in a number of diagnostic settings: (1) In the differential diagnosis of small B-cell lymphoma. Follicular lymphoma will show bcl-6 (and CD10) positivity whereas other small B-cell lymphomas are usually negative. (2) Bcl-6 is an important prognostic marker in diffuse large B-cell lymphomas (DLBCL), where CD10, bcl-6 and MUM1/IRF4 are used to identify germinal center and activated B-cell phenotypes. (3) Bcl-6 can be valuable in distinguishing classical Hodgkin lymphoma from nodular lymphocyte predominant Hodgkin lymphoma (NLPHL). The Reed-Sternberg cells of classical Hodgkin lymphoma are bcl-6 negative whereas the large ('LH') cells of NLPHL are bcl-6 positive. In contrast, anti-Bcl-6 rarely stains mantle-cell lymphoma and MALT lymphoma. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | BCL6 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Optimal dilution for a specific application should be determined. |
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| Theoretical MW | 95 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Bcl-6 Antibody (NBP2-59597)Find related products by research area.
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| Gene Symbol | BCL6 |
![Western Blot: Bcl-6 Antibody (BCL6/1475) - Azide and BSA Free [NBP2-59597] - Analysis (A) Recombinant Protein (B) HepG2 Cell lysate Using bcl-6 Monoclonal Antibody (BCL6/1475).](http://images.novusbio.com/fullsize/Bcl-6-Antibody-BCL6-1475-Azide-and-BSA-Free-Western-Blot-NBP2-59597-img0002.jpg "Western Blot: Bcl-6 Antibody (BCL6/1475) - Azide and BSA Free [NBP2-59597] - Analysis (A) Recombinant Protein (B) HepG2 Cell lysate Using bcl-6 Monoclonal Antibody (BCL6/1475).")
![Protein Array: Bcl-6 Antibody (BCL6/1475) - Azide and BSA Free [NBP2-59597] - Using more than 19000 full-length human proteins. Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary AB) produces when binding to a protein on the HuProt array. Z-scores are described in units of standard deviations above the mean value of all signals generated on that array. If targets on HuProt are arranged in descending order of the Z-score, the S-score is the difference between the Z-score. S-score therefore represents the relative target specificity of ab to its intended target. An Ab is considered specific to its intended target, if the Ab has an S-score of at least 2.5. For example, if Ab binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Ab to protein X is equal to 29.](http://images.novusbio.com/fullsize/Bcl-6-Antibody-BCL6-1475-Azide-and-BSA-Free-Protein-Array-NBP2-59597-img0001.jpg "Protein Array: Bcl-6 Antibody (BCL6/1475) - Azide and BSA Free [NBP2-59597] - Using more than 19000 full-length human proteins. Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary AB) produces when binding to a protein on the HuProt array. Z-scores are described in units of standard deviations above the mean value of all signals generated on that array. If targets on HuProt are arranged in descending order of the Z-score, the S-score is the difference between the Z-score. S-score therefore represents the relative target specificity of ab to its intended target. An Ab is considered specific to its intended target, if the Ab has an S-score of at least 2.5. For example, if Ab binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Ab to protein X is equal to 29.")
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