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Bcl-6 Antibody (BCL6/1475) [Alexa Fluor® 488]

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Bcl-6 Antibody (BCL6/1475) [Alexa Fluor® 488]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, MA, CyTOF-ready
Clone
BCL6/1475
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 488

Order Details

Bcl-6 Antibody (BCL6/1475) [Alexa Fluor® 488] Summary

Immunogen
Recombinant human bcl-6 protein fragment (around aa256-389) (exact sequence is proprietary) (Uniprot: P41182)
Localization
Nuclear
Marker
Follicular Lymphoma Marker
Specificity
The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. Recognizes a protein of 95kDa, which is identified as Bcl-6. Antibody to bcl-6 is helpful in a number of diagnostic settings: (1) In the differential diagnosis of small B-cell lymphoma. Follicular lymphoma will show bcl-6 (and CD10) positivity whereas other small B-cell lymphomas are usually negative. (2) Bcl-6 is an important prognostic marker in diffuse large B-cell lymphomas (DLBCL), where CD10, bcl-6 and MUM1/IRF4 are used to identify germinal center and activated B-cell phenotypes. (3) Bcl-6 can be valuable in distinguishing classical Hodgkin lymphoma from nodular lymphocyte predominant Hodgkin lymphoma (NLPHL). The Reed-Sternberg cells of classical Hodgkin lymphoma are bcl-6 negative whereas the large ('LH') cells of NLPHL are bcl-6 positive. In contrast, anti-Bcl-6 rarely stains mantle-cell lymphoma and MALT lymphoma.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
BCL6
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Protein Array
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Bcl-6 Antibody (BCL6/1475) [Alexa Fluor® 488]

  • B-cell CLL/lymphoma 6
  • B-cell lymphoma 6 protein transcript
  • Bcl-5
  • BCL5B-cell lymphoma 5 protein
  • Bcl6
  • Bcl-6
  • BCL6A
  • cys-his2 zinc finger transcription factor
  • LAZ3
  • LAZ3ZBTB27B-cell lymphoma 6 protein
  • lymphoma-associated zinc finger gene on chromosome 3
  • Protein LAZ-3
  • ZBTB27
  • Zinc finger and BTB domain-containing protein 27
  • Zinc finger protein 51ZNF51
  • zinc finger transcription factor BCL6S
  • ZNF51

Background

Bcl6 reacts with Bcl6 gene product in follicular lymphomas, diffuse large B cell lymphomas, Burkitt's lymphomas and in nodular, lymphocyte predominant Hodgkin's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol BCL6