![Immunoprecipitation: BCKDHA Antibody [NBP3-14681] - Whole cell lysate (1 mg per IP reaction, 20% of IP loaded) from Hep-G2 cells treated with phosphatase (+) or mock treated (-).Rabbit anti-BCKDHA antibody used for IP at 6 ug per reaction. For blotting immunoprecipitated rabbit anti-Phospho BCKDHA recombinant monoclonal antibody was used at 1:1000 (upper panel) was used at 1 ug/ml (lower panel). HRP-conjugated goat anti-rabbit IgG Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/BCKDHA-Antibody-Immunoprecipitation-NBP3-14681-img0001.jpg "Immunoprecipitation: BCKDHA Antibody [NBP3-14681] - Whole cell lysate (1 mg per IP reaction, 20% of IP loaded) from Hep-G2 cells treated with phosphatase (+) or mock treated (-).Rabbit anti-BCKDHA antibody used for IP at 6 ug per reaction. For blotting immunoprecipitated rabbit anti-Phospho BCKDHA recombinant monoclonal antibody was used at 1:1000 (upper panel) was used at 1 ug/ml (lower panel). HRP-conjugated goat anti-rabbit IgG Chemiluminescence with an exposure time of 30 seconds.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, Simple Western, IP |
| Clone | BLR060F |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Additional Information | Recombinant Monoclonal |
| Immunogen | surrounding serine 292 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | BCKDHA |
| Purity | >95% |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 2 - 8 C / 1 year from date of receipt |
| Buffer | Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and |
| Preservative | 0.09% Sodium Azide |
| Purity | >95% |
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Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | BCKDHA |
![Simple Western: BCKDHA Antibody [NBP3-14681] - Detection of mouse pBCKDHA (S292) by Simple Western™. Samples: Whole cell lysate (0.4 mg/mL) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho BCKDHA (S292) recombinant monoclonal antibody [BLR060F] (NBP3-14681) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally.](http://images.novusbio.com/fullsize/nbp3-14681_rabbit-bckdha-mab-simple-western-3032023111445.jpg "Simple Western: BCKDHA Antibody [NBP3-14681] - Detection of mouse pBCKDHA (S292) by Simple Western™. Samples: Whole cell lysate (0.4 mg/mL) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho BCKDHA (S292) recombinant monoclonal antibody [BLR060F] (NBP3-14681) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally.")
![Simple Western: BCKDHA Antibody [NBP3-14681] - Detection of human pBCKDHA (S292) by Simple Western™. Samples: Whole cell lysate (0.08 mg/mL) from HEK293T cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho BCKDHA (S292) recombinant monoclonal antibody [BLR060F] (NBP3-14681) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally.](http://images.novusbio.com/fullsize/nbp3-14681_rabbit-bckdha-mab-simple-western-3032023111648.jpg "Simple Western: BCKDHA Antibody [NBP3-14681] - Detection of human pBCKDHA (S292) by Simple Western™. Samples: Whole cell lysate (0.08 mg/mL) from HEK293T cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho BCKDHA (S292) recombinant monoclonal antibody [BLR060F] (NBP3-14681) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")