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Recombinant Human BAFFR/TNFRSF13C Protein

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SDS-Page: Recombinant Human BAFFR/TNFRSF13C Protein [NBP2-34879]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE, Bioactivity
Format
Carrier-Free
Concentration
LYOPH

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Recombinant Human BAFFR/TNFRSF13C Protein Summary

Description
A single non-glycosylated polypeptide chain containing 76 amino acids corresponding to BAFFR/TNFRSF13C Source: E. coli

Uniprot ID: Q96RJ3

Amino Acid Sequence: MRRGPRSLRG RDAPAPTPCV PAECFDLLVR HCVACGLLRT PRPKPAGASS PAPRTALQPQ ESVGAGAGEA ALPLPG

Details of Functionality
BAFF Receptor protein is fully biologically active when compared to standard. The ED50 as determined by its ability to block BAFF induced mouse splenocyte survival is 1.0-5.0 ug/ml in the presence of 1.0 ug/ml of rHuBAFF.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
TNFRSF13C
Purity
>95%, by reducing SDS-PAGE
Endotoxin Note
Less than 1 EU/ug of BAFFR/TNFRSF13C as determined by LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • SDS-Page
Theoretical MW
7.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 8.0, 500 mM NaCl.
Preservative
No Preservative
Concentration
LYOPH
Purity
>95%, by reducing SDS-PAGE
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Notes

This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions

Alternate Names for Recombinant Human BAFFR/TNFRSF13C Protein

  • BAFF R
  • BAFF Receptor/TNFRSF13C
  • BAFFR
  • BAFFR;BAFF-R;BROMIX;CD268;CVID4;prolixin;Tumor necrosis factor receptor superfamily member 13C
  • BR3
  • CD268
  • TNFRSF13C

Background

B Cell Activating Factor Receptor (BAFF-R), also named tumor necrosis factor receptor superfamily member 13C, is a member of the TNFR superfamily. It is highly expressed in spleen, lymph node, and resting B cells and to some extent in activated B cells, resting CD4+ cells and peripheral blood leukocytes. BAFF receptor is a type III transmembrane protein containing a single extracellular phenylalanine-rich domain and binds with high specificity to BAFF (TNFSF13B). It enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. BAFF receptor/BAFF signaling plays a critical role in B cell survival and maturation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol TNFRSF13C
Uniprot