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BAFFR/TNFRSF13C Antibody (2403C) [Unconjugated]

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Peripheral blood mononuclear cells (PBMCs) were stained with either (A) Rabbit Anti-Human BAFF R/TNFRSF13C Monoclonal Antibody (Catalog # MAB1162) or (B) Rabbit IgG Control Antibody (Catalog # MAB1050) followed by ...read more
BAFF R/TNFRSF13C was detected in immersion fixed paraffin-embedded sections of human spleen using Rabbit Anti-Human BAFF R/TNFRSF13C Monoclonal Antibody (Catalog # MAB1162) at 5 µg/mL for 1 hour at room ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, CyTOF-ready
Clone
2403C
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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BAFFR/TNFRSF13C Antibody (2403C) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Mouse myeloma cell line, NS0-derived human BAFF R/TNFRSF13C
Ser7-Ala71
Accession # Q96RJ3
Specificity
Detects human BAFF R/TNFRSF13C in direct ELISAs.
Source
N/A
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Purity Statement
Protein A or G purified from cell culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-25 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
MAB1162 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BAFFR/TNFRSF13C Antibody (2403C) [Unconjugated]

  • BAFF R
  • BAFFR
  • BAFFR;BAFF-R;BROMIX;CD268;CVID4;prolixin;Tumor necrosis factor receptor superfamily member 13C
  • BR3
  • CD268
  • TNFRSF13C

Background

B-cell activating factor (BAFF), also known as BlyS, TALL-1, TNAK, and zTNF4, is a TNF ligand superfamily member and has been designated TNFSF13B. Produced by macrophages, dendritic cells, and T lymphocytes, BAFF promotes the survival of B cells and is essential for B cell maturation (1-4). BAFF binds to three TNF receptor superfamily members: B-cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium-modulator and cyclophilin ligand interactor (TACI/TNFRSF13B) and BAFF receptor (BAFF R/BR3/TNFRSF13C). These receptors are type III transmembrane proteins that lack a signal peptide. Whereas TACI and BCMA bind BAFF and another TNF superfamily ligand, APRIL (a proliferation-inducing ligand), BAFF R selectively binds BAFF. The BAFF R extracellular domain lacks the TNF receptor canonical cysteine-rich domain (CRD) and contains only a partial CRD with four cysteine residues. Human and mouse BAFF R share 56% aa sequence identity. BAFF R is highly expressed in spleen, lymph node and resting B cells. It is also expressed at lower levels in activated B cell, in resting CD4+ T cells, in thymus and peripheral blood leukocytes. BAFF knockout mice lack mature B cells. Similarly, A/WySnJ mice that are defective in BAFF-R intracellular signaling also lack mature B cells, suggesting that BAFF R is the critical receptor for BAFF during B lymphopoiesis. In contrast, BCMA- or TACI-deficient mice have no major defect in B-cell development. While the function of BCMA is not defined, TACI has been shown to control B-cell homeostasis and T-cell-independent immune responses.

  1. Rolink, A.G. and F. Melcher (2002) Curr. Opin. Immunol. 14:266.
  2. Mackay F. and J.L. Browning (2002) Nature Reviews Immunology 2:464.
  3. Laabi, Y. et al. (2001) Current Biol. 11:R1013.
  4. Thompson, J.S. et al. (2001) Science 14:2108.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BAFFR/TNFRSF13C Antibody (MAB1162) (0)

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Review for BAFFR/TNFRSF13C Antibody (MAB1162) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB1162:
Filter by Applications
IHC-P
(1)
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(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin BAFFR/TNFRSF13C MAB1162
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4
reviewed by:
Lydia Taverne
IHC-P Human 12/16/2019
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman spleen, FFPE
SpeciesHuman
LotIRN0217041

Comments

CommentsCitrate buffer (pH 6) antigen unmasking. Antibody was used at a 1:50 concentration and detected with anti-rabbit AF546 (red fluor) secondary Ab.

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mFluor Violet Conjugated Antibodies

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Lydia Taverne
12/16/2019
Application: IHC-P
Species: Human

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