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BAF57 Antibody (1G10)

Images

 
Western Blot: BAF57 Antibody (1G10) [NBP3-43036] - Jurkat cells were subjected to SDS PAGE followed by western blot with (BAF57 Antibody) at dilution of 1:1000
Immunohistochemistry: BAF57 Antibody (1G10) [NBP3-43036] - Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using (BAF57 Antibody) at dilution of 1:100

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
1G10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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BAF57 Antibody (1G10) Summary

Immunogen
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1 - (Uniprot# Q969G3)
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SMARCE1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:20 - 1:200
  • Immunohistochemistry 1:20 - 1:200
  • Western Blot 1:500 - 1:2000
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A or G purified

Alternate Names for BAF57 Antibody (1G10)

  • BAF57BRG1-associated factor 57
  • chromatin remodeling complex BRG1-associated factor 57
  • subfamily e, member 1
  • SWI/SNF related, matrix associated, actin dependent regulator of chromatin
  • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin e1
  • SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily E member 1

Background

BAF57 is encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SMARCE1