Recombinant Human B7-H6 protein was serially diluted 2-fold and captured by Mouse Anti-Human B7-H6 Monoclonal Antibody (Catalog # MAB7144) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human B7-H6 ...read more
Genetic Strategies: B7-H6 knockout HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human B7-H6 Monoclonal Antibody (Catalog # MAB7144, filled histogram) or isotype control antibody ...read more
K562 cells were stained with Mouse Anti-Human B7‑H6 Monoclonal Antibody (Catalog # MAB7144, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by ...read more
Validation of cell surface proteins regulated by the US12 family.Flow cytometry confirmed proteomic data for proteins representative of each category enriched in the DAVID analysis. Staining in mock/US12 family member ...read more
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for B7-H6 Antibody (875001) [Unconjugated]
B7 homolog 6
B7H6
B7-H6
DKFZp686I21167
DKFZp686O24166
NCR3LG1
Background
B7-H6 is a glycosylated member of the B7 family of immune co‑stimulatory proteins (1, 2). Mature human B7-H6 consists of a 238 amino acid (aa) extracellular domain (ECD) that contains one Ig-like V domain and one Ig-like C1 domain, a 21 aa transmembrane segment, and a 171 aa cytoplasmic domain that contains one ITIM, one SH2, and one SH3 motif (3). Both of the Ig-like domains carry N-linked glycosylation (4). Within the ECD, human B7-H6 shares 99%, 94%, and 87% aa sequence identity with chimpanzee, orangutan, and gibbon B7-H6, respectively, and 53% - 56% with bovine, canine, and equine B7-H6. Orthologs in mouse and rat have not been identified. The Ig-like V domain mediates 1:1 stoichiometric binding of B7-H6 to NKp30 expressed on NK cells (4, 5). It does not show binding to NKp44, NKp46, or NKG2D (3, 6). Ligation of NKp30 by B7-H6 induces NK cell activation and target cell cytolysis (3). B7-H6 is expressed on a wide range of hematopoietic, carcinoma, and melanoma tumor cells, which is consistent with the detection of NKp30 binding sites on many tumors (3, 7). The expression of NKp30 ligands on tumor cells correlates with tumor cell sensitivity to NKp30‑dependent cell lysis (7).
Zou, W. and L. Chen (2008) Nat. Rev. Immunol. 8:467.
Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180.
Brandt, C.S. et al. (2009) J. Exp. Med. 206:1495.
Li, Y. et al. (2011) J. Exp. Med. 208:703.
Joyce, M.G. et al. (2011) Proc. Natl. Acad. Sci. 108:6223.
Arnon, T.I. et al. (2006) Semin. Cancer Biol. 16:348.
Byrd, A. et al. (2007) PLoS ONE 2:e1339.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for B7-H6 Antibody (MAB7144). (Showing 1 - 1 of 1 FAQs).
I would like to use this antibody on Western Blot. Do you have any experience with this antibody in this technique? I see that only Flow Cytometry and ELISA are described in the datasheet.
FYI, this antibody was produced by our sister company, R & D System. Unfortunately MAB7144 has never been tested in WB (only be validated in flow and ELISA) and therefore we don't know whether this antibody can work in the WB experiments; no protocol could be suggested to you, apologize for any inconvenience. Although we have another anti-human B7-H6 antibody, but it has only been validated in IHC-P (not WB). If you are willing to purchase and use either antibody in WB, you will be automatically qualified for our Innovator's Reward Program, which will award you the next antibody free of charge to you.
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