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B220/CD45R Antibody (HIS24)

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Immunohistochemistry-Frozen: B220/CD45R Antibody (HIS24) [NBP1-43408] - Staining of rat splenocytes with 0.5 ug of Mouse IgG2b K Isotype Control FITC (blue histogram) or 0.5 ug of Anti-Rat CD45R (B220) FITC (purple histogram). Cells in the lymphocyte gate were used for analysis (left). Immunohistochemistry of frozen rat spleen using 10 ug/mL Anti-Rat CD45R (B220) FITC (green), 10 ug/mL Anti-Rat Marginal Zone B Cells eFluor 660 (white), and 10 ug/mL Anti-Rat CD3 Biotin followed by 1 ug/mL Streptavidin eFluor (R) 570 (red). Nuclei are stained with DAPI (blue)(right).
Immunohistochemistry-Frozen: B220/CD45R Antibody (HIS24) [NBP1-43408] - Staining of rat splenocytes with 0.5 ug of Mouse IgG2b K Isotype Control FITC (blue histogram) or 0.5 ug of Anti-Rat CD45R (B220) FITC (purple ...read more
Flow Cytometry: B220/CD45R Antibody (HIS24) [NBP1-43408] - Analysis using the FITC conjugate of NBP1-43408. Staining of rat splenocytes with 0.05 ug of Mouse IgG2b K Isotype Control FITC (open histogram) or 0.05 ug of ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, Flow, IHC
Clone
HIS24
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-43408AF350
Alexa Fluor 405
NBP1-43408AF405
Alexa Fluor 488
NBP1-43408AF488
Alexa Fluor 532
NBP1-43408AF532
Alexa Fluor 594
NBP1-43408AF594
Alexa Fluor 647
NBP1-43408AF647
Alexa Fluor 700
NBP1-43408AF700
Alexa Fluor 750
NBP1-43408AF750
Allophycocyanin
NBP1-43408APC
Allophycocyanin/Cy7
NBP1-43408APCCY7
Biotin
NBP1-43408B
Biotin
NBP1-43637
CoraFluor 1
NBP1-43408CL1
DyLight 350
NBP1-43408UV
DyLight 405
NBP1-43408V
DyLight 488
NBP1-43408G
DyLight 550
NBP1-43408R
DyLight 594
NBP1-43408DL594
DyLight 650
NBP1-43408C
DyLight 680
NBP1-43408FR
DyLight 755
NBP1-43408IR
FITC
NBP1-44007
FITC
NBP1-43408F
HRP
NBP1-43408H
Janelia Fluor 525
NBP1-43408JF525
Janelia Fluor 549
NBP1-43408JF549
Janelia Fluor 585
NBP1-43408JF585
Janelia Fluor 635
NBP1-43408JF635
Janelia Fluor 646
NBP1-43408JF646
Janelia Fluor 669
NBP1-43408JF669
PE
NBP1-43408PE
PE/Atto594
NBP1-43408PEATT594
PE/Cy5.5
NBP1-43408PECY55
PE/Cy7
NBP1-43408PECY7
PerCP
NBP1-43408PCP
mFluor Violet 450 SE
NBP1-43408MFV450
mFluor Violet 500 SE
NBP1-43408MFV500
mFluor Violet 610 SE
NBP1-43408MFV610
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B220/CD45R Antibody (HIS24) Summary

Immunogen
The immunogen for this antibody was CD45R.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PTPRC
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Western Blot 1:100-1:2000

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Purity
Protein A or G purified

Alternate Names for B220/CD45R Antibody (HIS24)

  • B220
  • CD45 antigen
  • CD45R
  • EC 3.1.3.48
  • L-CA
  • LY5
  • protein tyrosine phosphatase, receptor type, C
  • receptor-type tyrosine-protein phosphatase C
  • T200 glycoprotein
  • T200 leukocyte common antigen
  • T200receptor type, c polypeptide

Background

The HIS24 monoclonal antibody reacts with an isoform of CD45 expressed by rat B cells. The antigen recognized by HIS24 is found on B cells from the early stage of B cell development in the bone marrow to mature peripheral B cells, but not on plasma cells. HIS24 recognizes a 205 kDa protein by immunoblotting. CD45, also known as Leukocyte Common Antigen (LCA), has tyrosine phosphatase enzymatic activity in its cytoplasmic portion and plays an important role in activation of lymphocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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B220/CD45R
Antibody (HIS24)
NBP1-43408
B220/CD45R Antibody (HIS24)
Species: Rt
Applications: WB, Flow, IHC

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PTPRC