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ATPase Na+/K+ beta 3 Antibody (M025)

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Western Blot: ATPase Na+/K+ beta 3 Antibody (M025) [NBP3-23434] - Western blot of native human MDA-MB-231 (lane 1), and MeWo (lane 2) cell lysates, as well as native human breast (lane 3), lung (lane 4), skin (lane 5), ...read more
Immunocytochemistry/ Immunofluorescence: ATPase Na+/K+ beta 3 Antibody (M025) [NBP3-23434] - Immunocytochemical labeling of ATPase Na+/K+ beta 3 in paraformaldehyde fixed human MeWo melanoma cells. The cells were ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IP
Clone
M025
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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ATPase Na+/K+ beta 3 Antibody (M025) Summary

Description
Stable for at least 1 year at -20°C.
Immunogen
Clone (M025) was generated from a proprietary antigen related to the native human ATPase Na+/K+ beta 3 subunit expressed in MeWo melanoma cell line. [UniProt# P54709]
Specificity
Clone M025 mouse monoclonal antibody detects a 40 kDa protein on SDS-PAGE "Native" immunoblots of human A431, LNCaP, MeWo, MDA-MB-231, and MCF7 cells. This antibody does not detect denatured ATPase Na+/K+ beta 3 subunit.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ATP1B3
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunoprecipitation 1:100
  • Western Blot 1:1000
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C.
Buffer
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Alternate Names for ATPase Na+/K+ beta 3 Antibody (M025)

  • ATP1B3
  • ATPase Na+/K+ beta 3
  • ATPase, Na+/K+ transporting, beta 3 polypeptide
  • ATPB-3
  • CD antigen CD298
  • CD298 antigen
  • CD298
  • FLJ29027
  • Na, K-ATPase beta-3 polypeptide
  • sodium/potassium-dependent ATPase beta-3 subunit
  • Sodium/potassium-dependent ATPase subunit beta-3
  • sodium/potassium-transporting ATPase beta-3 chain
  • sodium/potassium-transporting ATPase subunit beta-3

Background

ATPase Na+/ K+ beta 3 is encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 3 subunit. This gene encodes a beta 3 subunit. A pseudogene exists for this gene, and it is located on chromosome 2. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ATP1B3