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ATP6V1E1 Antibody

Images

 
Western Blot: ATP6V1E1 Antibody [NBP2-14335] - Analysis in human cell line SK-MEL-30.
Immunocytochemistry/ Immunofluorescence: ATP6V1E1 Antibody [NBP2-14335] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: ATP6V1E1 Antibody [NBP2-14335] - Analysis in human cerebral cortex and skeletal muscle tissues using NBP2-14335 antibody. Corresponding ATP6V1E1 RNA-seq data are presented for the same ...read more
Immunohistochemistry-Paraffin: ATP6V1E1 Antibody [NBP2-14335] - Staining of human cerebral cortex shows moderate granular cytoplasmic positivity in neuropil.
Immunohistochemistry-Paraffin: ATP6V1E1 Antibody [NBP2-14335] - Staining of human kidney shows strong membranous positivity in cells in tubules.
Immunohistochemistry-Paraffin: ATP6V1E1 Antibody [NBP2-14335] - Staining of human pancreas shows moderate cytoplasmic positivity in islets of Langerhans.
Immunohistochemistry-Paraffin: ATP6V1E1 Antibody [NBP2-14335] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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ATP6V1E1 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the amino acids: KEKQIEQQKKIQMSNLMNQARLKVLRARDDLITDLLNEAKQRLSKVVKDTTRYQVLLDGLVLQGLYQLLEPRMIVRCRKQDFPLVKAAVQKAIPMYKIATKNDVDVQIDQESYLPEDIAGGVEIYNGDRKIKVSNTLESRLDLIAQQMMPEVRGALFGANANRKF
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATP6V1E1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
ATP6V1E1 Protein (NBP2-14335PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ATP6V1E1 Antibody

  • ATP6E2ATP6V1E
  • ATP6E31kDa subunit
  • ATPase, H+ transporting, lysosomal (vacuolar proton pump) 31kD
  • ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E isoform 1
  • ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E1
  • H+-transporting ATP synthase chain E, vacuolar
  • p31
  • Vacuolar proton pump subunit E 1
  • V-ATPase 31 kDa subunit
  • V-ATPase subunit E 1
  • V-ATPase, subunit E
  • Vma4
  • V-type proton ATPase subunit E 1

Background

ATP6V1E1 encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. This gene encodes alternate transcriptional splice variants, encoding different V1 domain E subunit isoforms. Pseudogenes for this gene have been found in the genome. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol ATP6V1E1