ATM Antibody (5C2)

ATM (ataxia telangiectasia mutated kinase) is the master regulator of the DNA double-strand break (DSB) repair pathway. This ubiquitously expressed serine/threonine protein kinase belongs to the PI3K-like family of proteins and responds to DSBs caused by oxidative and other genotoxic stresses (1). In addition to regulating the DNA damage response, ATM participates in vesicle and protein transport, T-cell development, gonads/neurological function, pre-B cell allelic exclusion, cell cycle control, and acts as a tumor suppressor (2,3). Defects in ATM are associated with ataxia telangiectasia (AT), T-cell acute lymphoblastic leukemia (TALL), T-prolymphocytic leukemia (TPLL), and B-cell non-Hodgkin lymphomas (BNHL) including mantle cell lymphoma (MCL) and B-cell chronic lymphocytic leukemia (BCLL) (4).

The theoretical molecular weight of ATM is 350 kDa and it has 3 main domains: a FAT (focal adhesion targeting) domain (aa 1960-2566), a PI-3/PI-4 kinase catalytic domain (aa 2712-2962), and a C-terminal FAT domain (aa 3024-3056). ATM exists as a dimer or tetramer in its inactive state. Upon sensing DNA damage, the MRE11-RAD50-NBS1 (MRN) complex recruits ATM. The intricate process of ATM activation involves acetylation by KAT5/TIP60, autophosphorylation at Ser-1981, and dissociation into catalytically active monomers (5). Following activation, ATM phosphorylates multiple substrates such as p53/TP53 and Chk2 involved in DNA repair, checkpoint signaling, and the apoptosis pathway.

References

1. Paull TT. (2015) Mechanisms of ATM Activation. Annu Rev Biochem. 84:711-38. PMID: 25580527

2. Chaudhary MW and Al-Baradie RS. (2014) Ataxia-telangiectasia: future prospects. Appl Clin Genet. 7:159-167. PMID: 25258552

3. Stagni V, Cirotti C, and Barila D. (2018) Ataxia-Telangiectasia Mutated Kinase in the Control of Oxidative Stress, Mitochondria, and Autophagy in Cancer: A Maestro With a Large Orchestra. Front Oncol. 8:73. PMID: 29616191

4. Gumy-Pause F, Wacker P, and Sappino AP. (2004) ATM gene and lymphoid malignancies. Leukemia. 18(2):238-42. PMID: 14628072

5. Adamowicz M. (2018) Breaking up with ATM. J Immunol Sci. 2(1):26-31. PMID: 29652413

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Chicken.

We have publications tested in 2 applications: ICC/IF, WB.

Showing Publications 1 - 10 of 35. Show All 35 Publications. Collapse Publications.

Publications using NB100-220	Applications	Species
Athanasios Siametis, Kalliopi Stratigi, Despoina Giamaki, Georgia Chatzinikolaou, Alexia Akalestou-Clocher, Evi Goulielmaki, Brian Luke, Björn Schumacher, George A. Garinis Transcription stress at telomeres leads to cytosolic DNA release and paracrine senescence Nature Communications 2024-05-14 [PMID: 38744897]
Xiaozhen Zhang, Mengyi Lao, Hanshen Yang, Kang Sun, Yunfei Dong, Lihong He, Xinchi Jiang, Honghui Wu, Yangwei Jiang, Muchun Li, Honggang Ying, Xinyuan Liu, Jian Xu, Yan Chen, Hanjia Zhang, Ruhong Zhou, Jianqing Gao, Xueli Bai, Tingbo Liang Targeting cancer-associated fibroblast autophagy renders pancreatic cancer eradicable with immunochemotherapy by inhibiting adaptive immune resistance Autophagy 2024-01-04 [PMID: 38174993]
Yuan J, Zhao F, Liu Y et al. Effects of Lactiplantibacillus plantarum on oxidative stress, mitophagy, and NLRP3 inflammasome activation in broiler breast meat Poultry Science 2023-09-20 [PMID: 37832190] (WB, Chicken)	WB	Chicken
S Mukhopadhy, J Encarnació, EY Lin, ASW Sohn, H Zhang, JD Mancias, AC Kimmelman Autophagy supports mitochondrial metabolism through the regulation of iron homeostasis in pancreatic cancer Science Advances, 2023-04-19;9(16):eadf9284. 2023-04-19 [PMID: 37075122]
Najnin R, Al Mahmud M, Rahman M et al. ATM suppresses c-Myc overexpression in the mammary epithelium in response to estrogen Cell Reports 2023-01-01 [PMID: 36640339] (ICC/IF, Mouse, Human)	ICC/IF	Mouse, Human
Trudu M, Oliva L, Orfanelli U et al. Preclinical evidence of a direct pro-survival role of arginine deprivation in multiple myeloma Frontiers in Oncology 2022-09-08 [PMID: 36172163] (WB)	WB
Nollet EA, Cardo-Vila M, Ganguly SS et al. Androgen receptor-induced integrin alpha 6 beta 1 and Bnip3 promote survival and resistance to PI3K inhibitors in castration-resistant prostate cancer Oncogene 2020-06-21 [PMID: 32565538]
Thayer, JA;Awad, O;Hegdekar, N;Sarkar, C;Tesfay, H;Burt, C;Zeng, X;Feldman, RA;Lipinski, MM; The PARK10 gene USP24 is a negative regulator of autophagy and ULK1 protein stability Autophagy 2019-04-07 [PMID: 30957634]
Meister-Broekema M, Freilich R, Jagadeesan C et al. Myopathy associated BAG3 mutations lead to protein aggregation by stalling Hsp70 networks Nat Commun 2018-12-17 [PMID: 30559338] (ICC/IF, Human)	ICC/IF	Human
Barnard RA, Regan DP, Hansen RJ et al. Autophagy Inhibition Delays Early but Not Late-Stage Metastatic Disease. J Pharmacol Exp Ther 2016-08-01 [PMID: 27231155]
Aumailley L, Garand C, Dubois MJ et al. Metabolic and Phenotypic Differences between Mice Producing a Werner Syndrome Helicase Mutant Protein and Wrn Null Mice. PLoS One 2015-01-01 [PMID: 26447695] (ICC/IF, Mouse)	ICC/IF	Mouse
Milan E, Perini T, Resnati M et al. A plastic SQSTM1/p62-dependent autophagic reserve maintains proteostasis and determines proteasome inhibitor susceptibility in multiple myeloma cells. Autophagy 2015-01-01 [PMID: 26043024] (WB)	WB
Mendez G, Cilli D, Berardinelli F et al. Cleavage of the BRCT tandem domains of nibrin by the 657del5 mutation affects the DNA damage response less than the Arg215Trp mutation IUBMB Life 2012-10-01 [PMID: 22941933]
Daniel JA, Pellegrini M, Lee BS et al. Loss of ATM kinase activity leads to embryonic lethality in mice J Cell Biol 2012-08-06 [PMID: 22869595] (WB, Mouse)	WB	Mouse
Hou YY, Toh MT, Wang X. NBS1 deficiency promotes genome instability by affecting DNA damage signaling pathway and impairing telomere integrity. Cell Biochemistry and Function. 2011-12-09 [PMID: 22161642]
Vogel R, Seyffert M, Strasser R et al. Adeno-associated virus type 2 modulates the host DNA damage response induced by herpes simplex virus 1 during coinfection. J Virol 2012-01-01 [PMID: 22013059] (ICC/IF, Human)	ICC/IF	Human
Rankin AM, Sarkar S, Faller DV et al. Mechanism of T-oligo-induced cell cycle arrest in Mia-PaCa pancreatic cancer cells. J Cell Physiol 2012-06-01 [PMID: 21898405] (ICC/IF, Human)	ICC/IF	Human
Gapud EJ, Dorsett Y, Yin B et al. Ataxia telangiectasia mutated (Atm) and DNA-Pkcs kinases have overlapping activities during chromosomal signal joint formation. Proc Natl Acad Sci USA 2011-02-01 [PMID: 21245316] (ICC/IF, Human)	ICC/IF	Human
Cam H, Easton JB, High A et al. mTORC1 signaling under hypoxic conditions is controlled by ATM-dependent phosphorylation of HIF-1a. Mol Cell 2010-11-01 [PMID: 21095582] (ICC/IF, Human)	ICC/IF	Human
Craig AL, Holcakova J, Finlan LE et al. DeltaNp63 transcriptionally regulates ATM to control p53 Serine-15 phosphorylation. Mol Cancer 2010-07-01 [PMID: 20663147] (ICC/IF, Human)	ICC/IF	Human
Alimonti A, Nardella C, Chen Z et al. A novel type of cellular senescence that can be enhanced in mouse models and human tumor xenografts to suppress prostate tumorigenesis. J Clin Invest 2010-03-01 [PMID: 20197621]
Gonzalez-Suarez I, Redwood AB, Perkins SM et al. Novel roles for A-type lamins in telomere biology and the DNA damage response pathway. EMBO J 2009-08-01 [PMID: 19629036] (ICC/IF, Human)	ICC/IF	Human
Efeyan A, Murga M, Martinez-Pastor B et al. Limited role of murine ATM in oncogene-induced senescence and p53-dependent tumor suppression. PLoS One 2009-01-01 [PMID: 19421407] (WB, Mouse)	WB	Mouse
Siehler SY, Schrauder M, Gerischer U et al. Human MutL-complexes monitor homologous recombination independently of mismatch repair. DNA Repair (Amst) 2009-02-01 [PMID: 19022408] (ICC/IF, Human)	ICC/IF	Human
Ariumi Y, Kuroki M, Dansako H et al. The DNA damage sensors ataxia-telangiectasia mutated kinase and checkpoint kinase 2 are required for hepatitis C virus RNA replication. J Virol 2008-10-01 [PMID: 18667510] (ICC/IF, Human)	ICC/IF	Human
Sun Y, Xu Y, Roy K et al. DNA damage-induced acetylation of lysine 3016 of ATM activates ATM kinase activity. Mol Cell Biol 2007-12-01 [PMID: 17923702] (ICC/IF, Human)	ICC/IF	Human
Fernandes ND, Sun Y, Price BD. Activation of the kinase activity of ATM by retinoic acid is required for CREB-dependent differentiation of neuroblastoma cells. J Biol Chem 2007-06-01 [PMID: 17426037] (ICC/IF, Human)	ICC/IF	Human
Chen JH, Ozanne SE. Deep senescent human fibroblasts show diminished DNA damage foci but retain checkpoint capacity to oxidative stress. FEBS Lett 2006-12-01 [PMID: 17126333] (ICC/IF, Human)	ICC/IF	Human
Sun Y, Jiang X, Chen S et al. Inhibition of histone acetyltransferase activity by anacardic acid sensitizes tumor cells to ionizing radiation. FEBS Lett 2006-08-01 [PMID: 16844118] (ICC/IF, Human)	ICC/IF	Human
Roy K, Wang L, Makrigiorgos GM et al. Methylation of the ATM promoter in glioma cells alters ionizing radiation sensitivity. Biochem Biophys Res Commun 2006-06-01 [PMID: 16631604] (ICC/IF, Human)	ICC/IF	Human
Jiang X, Sun Y, Chen S et al. The FATC domains of PIkk proteins are functionally equivalent and participate in the Tip60-dependent activation of DNA-Pkcs and ATM. J Biol Chem 2006-06-01 [PMID: 16603769] (ICC/IF, Human)	ICC/IF	Human
Shiotani B, Kobayashi M, Watanabe M et al. Involvement of the ATR- and ATM-dependent checkpoint responses in cell cycle arrest evoked by pierisin-1. Mol Cancer Res 2006-02-01 [PMID: 16513843] (ICC/IF, Human)	ICC/IF	Human
Sun Y, Jiang X, Chen S et al. A role for the Tip60 histone acetyltransferase in the acetylation and activation of ATM. Proc Natl Acad Sci USA 2005-09-01 [PMID: 16141325] (ICC/IF, Human)	ICC/IF	Human
Fernandes N, Sun Y, Chen S et al. DNA damage-induced association of ATM with its target proteins requires a protein interaction domain in the N terminus of ATM. J Biol Chem 2005-04-01 [PMID: 15713674] (ICC/IF, Human)	ICC/IF	Human
Lai CH, Chun HH, Nahas SA et al. Correction of ATM gene function by aminoglycoside-induced read-through of premature termination codons. Proc Natl Acad Sci USA 2004-11-01 [PMID: 15498871] (ICC/IF, Human)	ICC/IF	Human
Show All 35 Publications. Collapse Publications.

Array NB100-220

• ATM Antibodies
• ATM ELISA Kits
• ATM Proteins