Raji Whole Cell Lysate Summary
| Description |
We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use. |
| Specificity |
Total Protein - Human Tumor Cell Line: Raji |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression |
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles. |
| Buffer |
HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors |
| Concentration |
5.0 mg/ml |
Lysate Details for Array
| Type |
Cell |
| Tissue |
Blood Lymph |
| Protein State |
Native |
| Subcellular Fraction |
Whole |
Background
Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are
guaranteed for 6 months from date of receipt.
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
Video Protocols
FAQs for Raji Lysate (NB820-59466). (Showing 1 - 1 of 1 FAQ).
-
Were the cells lysed immediately after collection, or was the cell pellet frozen and lysed at a later date?
- The lysates are prepared from frozen cell pellets.
Additional Raji Products
Blogs on Raji
![Western Blot: Raji Whole Cell Lysate [NB820-59466] - Protein lysate can be validated by Western Blot using specific biomarker: GAPDH for total protein, cytoplasmic and universal total protein; Histone for nuclear protein; Na/K ATPase for membrane protein; Vimentin for cytoskeleton protein.](http://images.novusbio.com/fullsize/Raji-Whole-Cell-Lysate-Western-Blot-NB820-59466-img0002.jpg "Western Blot: Raji Whole Cell Lysate [NB820-59466] - Protein lysate can be validated by Western Blot using specific biomarker: GAPDH for total protein, cytoplasmic and universal total protein; Histone for nuclear protein; Na/K ATPase for membrane protein; Vimentin for cytoskeleton protein.")
![SDS-Page: Raji Whole Cell Lysate [NB820-59466] - Protein lysate shows consistent pattern.](http://images.novusbio.com/fullsize/Raji-Whole-Cell-Lysate-SDS-Page-NB820-59466-img0001.jpg "SDS-Page: Raji Whole Cell Lysate [NB820-59466] - Protein lysate shows consistent pattern.")