ASGPR1 Antibody (8D7)

The asialoglycoprotein (ASGP) receptor is a transmembrane hepatocellular surface carbohydrate binding glycoproteins lacking terminal sialic acid residues (asialoglycoproteins). Characterization of the ASGP receptor revealed its functional role in the binding, internalization and transport of a wide range of glycoproteins, which have exposed galactose or N-acetylgalactosamine residues, via the process of receptor-mediated endocytosis (RME). The ASGP receptor can bind a variety of important plasma proteins including transport proteins (i.e. transferrin), enzymes such as alkaline phosphatase, immunoglobulins including IgA, apoptotic hepatocytes, fibronectin and platelets. Additionally, the expression of the ASGP receptor has been clinically correlated to the level of hepatic function that is lost during liver diseases related to cancer, viral hepatitis, and cirrhosis. The ASGP receptor consists of major and minor subunits, which in the rat were identified as rat hepatic lectin (RHL) 1 and RHL 2/3, with molecular weights of respectively 42, 49 and 54 kDa. The selective binding (calcium and pH depended) and uptake of terminal galactosyl bearing proteins requires the formation of hetero-oligomers between these major and minor forms The total ASGP receptor population consisted of two functionally distinct receptor populations, designated State 1 and State 2, which were involved in the endocytosis and intracellular processing of ligands by different pathways. The monoclonal antibody 8D7 recognizes a subunit-specific epitope on RHL-1 of rat ASGPR. The monoclonal antibody 8D7 is cross reactive with human ASGPR.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: Flow Cytometry, WB, Western Blot.

Showing Publications 1 - 10 of 11. Show All 11 Publications. Collapse Publications.

Publications using NBP1-60150	Applications	Species
Chander N, Basha G, Yan Cheng M et al. Lipid nanoparticle mRNA systems containing high levels of sphingomyelin engender enhanced protein expression in hepatic and extra-hepatic tissues Molecular Therapy - Methods & Clinical Development 2023-06-11 [PMID: 37564393] (Flow Cytometry, Mouse)	Flow Cytometry	Mouse
Carol C, Amanda M, Ryan G et al. Alcohol-Induced Liver Injury: Down-regulation and Redistribution of Rab3D Results in Atypical Protein Trafficking Hepatol Commun. 2022-02-06 [PMID: 34494400] (Western Blot, Human)	Western Blot	Human
Demina EP, Smutova V, Pan X et al. Neuraminidases 1 and 3 Trigger Atherosclerosis by Desialylating Low-Density Lipoproteins and Increasing Their Uptake by Macrophages Journal of the American Heart Association 2021-02-16 [PMID: 33554615] (WB, Mouse)	WB	Mouse
Kiamehr M, Viiri L E et al. Lipidomic profiling of patient-specific iPSC-derived hepatocyte-like cells. Dis Model Mech 2017-01-09 [PMID: 28733363] (Mouse)		Mouse
Zhou XL, Sun PN, Huang TH, Xie QD, Kang XJ, Liu LM. Effects of hepatitis B virus S protein on human sperm function. Hum Reprod;24(7):1575-83. 2009-07-01 [PMID: 19279032]
Keyel PA, Mishra SK, Roth R, Heuser JE, Watkins SC, Traub LM. A single common portal for clathrin-mediated endocytosis of distinct cargo governed by cargo-selective adaptors. Mol Biol Cell;17(10):4300-17. 2006-10-01 [PMID: 16870701]
Shimada M, Mizuno M, Uesu T, Nasu J, Okada H, Shimomura H, Yamamoto K, Tsuji T, Shiratori Y. A monoclonal antibody to rat asialoglycoprotein receptor that recognizes an epitope specific to its major subunit. Hepatol Res. 26(1):55-60. 2003-05-01 [PMID: 12787805]
Hirai M, Mizuno M, Morisue Y, Yoshioka M, Shimada M, Nasu J, Okada H, Shimomura H, Yamamoto K, Tsuji T. Development of syngeneic monoclonal anti-idiotype antibodies to mouse monoclonal anti-asialoglycoprotein receptor antibody. Source Department of Medicine and Medical Science, Okayama University Graduate School of Medicine and Dentistry, Japan . Acta Med Okayama. 56(3):135-9. 2002-06-01 [PMID: 12108584]
Hyodo I, Mizuno M, Yamada G, Tsuji T. Distribution of asialoglycoprotein receptor in human hepatocellular carcinoma. Liver;13(2):80-5. 1993-04-01 [PMID: 8389955]
Mizuno M, Yamada G, Sugiyama T, Vierling JM, Brown WR. Monoclonal antibodies identifying antigens on distinct domains of rat hepatocytes. Liver;7(5):251-9. 1987-10-01 [PMID: 3695813]
Mizuno M, Yamada G, Nagashima H. Development of a monoclonal antibody identifying an antigen which is segregated to the sinusoidal and lateral plasma membranes of rat hepatocytes. Gastroenterol Jpn;21(3):238-44. 1986-06-01 [PMID: 3525313]
Show All 11 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
	4 reviewed by: Armen Petrosyan	IF	Mouse	10/06/2021	View Summary Application Immunofluorescence Sample Tested Mouse Liver cells Species Mouse Lot 1

Array NBP1-60150

• ASGPR1 Antibodies
• ASGPR1 Antibody Pair
• ASGPR1 ELISA Kits
• ASGPR1 Lysate
• ASGPR1 Proteins
• ASGPR1 Proteins and Enzymes