![Immunohistochemistry-Frozen: ARID5A Antibody (18112) [NBP2-43745] - Sectioned mouse mouse cerebellum. Green: ARID5A stained by ARID5A antibody [18112] diluted at 1:250. Red: Calbindin, stained by Calbindin antibody diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 10 min.](http://images.novusbio.com/fullsize/ARID5A-Antibody-18112-Immunohistochemistry-Frozen-NBP2-43745-img0005.jpg "Immunohistochemistry-Frozen: ARID5A Antibody (18112) [NBP2-43745] - Sectioned mouse mouse cerebellum. Green: ARID5A stained by ARID5A antibody [18112] diluted at 1:250. Red: Calbindin, stained by Calbindin antibody diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 10 min.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, Flow, IHC |
| Clone | 18112 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ARID5A. The exact sequence is proprietary. |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ARID5A |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Theoretical MW | 64 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
|
| Reviewed Applications |
|
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS, 20% Glycerol |
| Preservative | No Preservative |
| Purity | Protein A purified |
![Immunohistochemistry Melanotransferrin/CD228/MFI2 Antibody (893438) [Unconjugated]](https://images.novusbio.com/images2/Melanotransferrin_MAB8175_Immunohistochemistry_21715.jpg)
![Simple Western Melanotransferrin/CD228/MFI2 Antibody (893438) [Unconjugated]](https://images.novusbio.com/images2/Melanotransferrin_MAB8175_Simple_Western_17260.jpg)
![Western Blot Melanotransferrin/CD228/MFI2 Antibody (893438) [Unconjugated]](https://images.novusbio.com/images2/Melanotransferrin_MAB8175_Western_Blot_14484.jpg)
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Enlarge |
reviewed by:
Verified Customer |
WB | Human | 05/07/2020 |
Summary
Comments
|
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | ARID5A |
![Flow Cytometry: ARID5A Antibody (18112) [NBP2-43745] - ARID5A antibody [18112] detects ARID5A protein by flow cytometry analysis. Sample: THP-1 cell. Black: Unlabelled sample was used as a control.Red: ARID5A antibody [18112] dilution: 1:20. Acquisition of 20,000 events were collected using a DyLight 488-conjugated secondary antibody for FACS analysis.](http://images.novusbio.com/fullsize/ARID5A-Antibody-18112-Flow-Cytometry-NBP2-43745-img0004.jpg "Flow Cytometry: ARID5A Antibody (18112) [NBP2-43745] - ARID5A antibody [18112] detects ARID5A protein by flow cytometry analysis. Sample: THP-1 cell. Black: Unlabelled sample was used as a control.Red: ARID5A antibody [18112] dilution: 1:20. Acquisition of 20,000 events were collected using a DyLight 488-conjugated secondary antibody for FACS analysis.")
![Western Blot: ARID5A Antibody (18112) [NBP2-43745] - Analysis of Non-transfected (-) and ARID5A-transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ARID5A antibody diluted by 1:5000.](http://images.novusbio.com/fullsize/ARID5A-Antibody-18112-Western-Blot-NBP2-43745-img0001.jpg "Western Blot: ARID5A Antibody (18112) [NBP2-43745] - Analysis of Non-transfected (-) and ARID5A-transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ARID5A antibody diluted by 1:5000.")
![Western Blot: ARID5A Antibody (18112) [NBP2-43745] - Untreated (-) and treated (+) Raw264.7 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ARID5A antibody 18112]. The signal was developed with Trident ECL plus-Enhanced. (LPS: Lipopolysaccharides)](http://images.novusbio.com/fullsize/ARID5A-Antibody-18112-Western-Blot-NBP2-43745-img0002.jpg "Western Blot: ARID5A Antibody (18112) [NBP2-43745] - Untreated (-) and treated (+) Raw264.7 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ARID5A antibody 18112]. The signal was developed with Trident ECL plus-Enhanced. (LPS: Lipopolysaccharides)")
![Western Blot: ARID5A Antibody (18112) [NBP2-43745] - THP-1 whole cell and nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ARID5A antibody [18112] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/antibody/nbp2-43745_mouse-monoclonal-arid5a-antibody-18112-western-blot-342024103558..jpg "Western Blot: ARID5A Antibody (18112) [NBP2-43745] - THP-1 whole cell and nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ARID5A antibody [18112] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Immunocytochemistry S100A8 Antibody [Unconjugated]](https://images.novusbio.com/images2/S100A8_AF3059_Immunocytochemistry_6489.jpg)
![Western Blot S100A8 Antibody [Unconjugated]](https://images.novusbio.com/images2/S100A8_AF3059_Immunocytochemistry__Immunofluorescence_17308.jpg)
![Simple Western S100A8 Antibody [Unconjugated]](https://images.novusbio.com/images2/S100A8_AF3059_Simple_Western_18694.jpg)
![Immunocytochemistry SOX9 Antibody [Unconjugated]](https://images.novusbio.com/images2/SOX9_AF3075_Immunocytochemistry_15995.jpg)
![Western Blot SOX9 Antibody [Unconjugated]](https://images.novusbio.com/images2/SOX9_AF3075_Western_Blot_21580.jpg)
![Immunocytochemistry SOX9 Antibody [Unconjugated]](https://images.novusbio.com/images2/SOX9_AF3075_Immunocytochemistry_6490.jpg)
