Home » Melanotransferrin/CD228/MFI2 » Melanotransferrin/CD228/MFI2 Antibodies » Melanotransferrin/CD228/MFI2 Antibody (893438) [Unconjugated]
Novus Biologicals products are now on bio-techne.com

Melanotransferrin/CD228/MFI2 Antibody (893438) [Unconjugated]

 
 Submit a Review

Images

 
(
Enlarge)
Western blot shows lysates of SK-Mel-28 human malignant melanoma cell line and Hs 294T human melanoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Melanotransferrin/CD228 Monoclonal Antibody (Catalog # MAB8175) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Melanotransferrin/CD228 at approximately 90-95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of SK-Mel-28 human malignant melanoma cell line and Hs 294T human melanoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Melanotransferrin/CD228 Monoclonal Antibody ...read more
Melanotransferrin/CD228 was detected in perfusion fixed frozen sections of human melanoma tissue using Mouse Anti-Human Melanotransferrin/CD228 Monoclonal Antibody (Catalog # MAB8175) at 5 µg/mL for 1 hour at ...read more
Simple Western lane view shows lysates of SK‑Mel‑28 human malignant melanoma cell line, loaded at 0.5 mg/mL. A specific band was detected for Melanotransferrin/CD228 at approximately 109 kDa (as indicated) using 10 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC
Clone
893438
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB8175-SP
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Melanotransferrin/CD228/MFI2 Antibody (893438) [Unconjugated] Summary

Immunogen
HEK293 human embryonic kidney cell line transfected with human Melanotransferrin/CD228
Met1-Gly711
Accession # P08582
Specificity
Detects human Melanotransferrin/CD228 in ELISAs.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MFI2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-25 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Melanotransferrin/CD228/MFI2 Antibody (893438) [Unconjugated]

  • antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and96.5
  • CD228 antigen
  • CD228
  • FLJ38863
  • MAP97
  • MAP97Melanoma-associated antigen p97
  • Melanotransferrin
  • membrane-bound transferrin-like protein
  • MFI2
  • MGC4856
  • MTF
  • MTF1
  • p97

Background

Melanotransferrin, also known as MTF, CD228, melanoma-associated antigen p97, MAP97 and MFI2, is a 90-97 kDa sialoglycoprotein member of the transferrin family. Unlike other transferrins, which are secreted, MTF is usually found tethered to the cell membrane by a glycosyl phosphatidyl inositol anchor, with only small amounts of soluble protein detected. MTF is highly expressed on melanoma cells, and at lower levels in salivary gland, pancreas, kidney and testis. Like other transferrins, MTF is an iron-binding protein, and may play roles in cellular proliferation, tumorigenesis, metastasis and migration. Full-length human, mouse and rat MTF is 738 amino acids (aa). Over aa 1-711, human MTF shares 85 and 86% aa identity with mouse and rat MTF, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Holo-Transferrin
[Unconjugated]
2914-HT
Holo-Transferrin [Unconjugated]
(51 Publications)
Species: Hu
Applications: BA
Gemin 4 Antibody
NB110-40591
Western Blot Gemin 4 Antibody
Immunohistochemistry-Paraffin Gemin 4 Antibody
(1 Publication)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, IP, WB
p97/VCP Antibody
NB100-1558
Western Blot p97/VCP Antibody
Western Blot p97/VCP Antibody
Western Blot p97/VCP Antibody
(12 Publications)
Species: Hu, Mu, Rt, Ze
Applications: ICC/IF, IHC, IHC-Fr, IP, WB
Craniofacial
Development Protein 1 ...
NBP2-43772
Immunohistochemistry-Paraffin Craniofacial Development Protein 1 Antibody (11811)
Immunohistochemistry-Paraffin Craniofacial Development Protein 1 Antibody (11811)
Immunocytochemistry/ Immunofluorescence Craniofacial Development Protein 1 Antibody (11811)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Proteasome 19S 10B
Antibody
NB100-1483
Western Blot Proteasome 19S 10B Antibody
Immunocytochemistry/ Immunofluorescence Proteasome 19S 10B Antibody
Flow Cytometry Proteasome 19S 10B Antibody
(6 Publications)
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, PEP-ELISA, PLA, WB
DAP5 Antibody
NBP1-85311
Western Blot DAP5 Antibody
Western Blot DAP5 Antibody
Immunohistochemistry-Paraffin DAP5 Antibody
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KD, WB
Ferritin
mitochondrial Antibod
NBP2-14961
Western Blot Ferritin mitochondrial Antibody
Immunohistochemistry-Paraffin Ferritin mitochondrial Antibody
Species: Hu, Mu
Applications: IHC, IHC-P, WB
Lactoferrin
Antibody (1C6cc)
NB120-10109
CyTOF-ready Lactoferrin Antibody (1C6cc)
(1 Review)
(1 Publication)
Species: Hu
Applications: CyTOF-ready, IHC, IHC-P, S-ELISA, WB
Plasminogen
Antibody
NB600-930
Western Blot Plasminogen Antibody
Western Blot Plasminogen Antibody
Western Blot Plasminogen Antibody
(2 Reviews)
(6 Publications)
Species: Hu, Rt
Applications: ELISA, WB
MTRF1 Antibody
H00009617-B01P
Western Blot MTRF1 Antibody
Western Blot MTRF1 Antibody
(1 Publication)
Species: Hu
Applications: WB
c-jun [p Ser63]
Antibody (1018Y) [Unc ...
MAB8930
Western Blot c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
Immunocytochemistry c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
Simple Western c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
(1 Review)
(3 Publications)
Species: Hu
Applications: ICC, Simple Western, WB
ARID5A Antibody
(18112)
NBP2-43745
Flow Cytometry ARID5A Antibody (18112)
Immunohistochemistry-Frozen ARID5A Antibody (18112)
Western Blot ARID5A Antibody (18112)
(1 Review)
Species: Hu, Mu
Applications: Flow, IHC, IHC-Fr, WB
Adenine Nucleotide
Translocator 2 Antibo ...
NBP2-92630
Immunocytochemistry/ Immunofluorescence Adenine Nucleotide Translocator 2 Antibody - Azide and BSA Free
Immunohistochemistry-Paraffin Adenine Nucleotide Translocator 2 Antibody - Azide and BSA Free
Western Blot Adenine Nucleotide Translocator 2 Antibody - Azide and BSA Free
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
TfR (Transferrin
R) Antibody - BSA Fre
NB100-92243
Immunohistochemistry-Paraffin TfR (Transferrin R) Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence TfR (Transferrin R) Antibody - BSA Free
Western Blot TfR (Transferrin R) Antibody - BSA Free
(1 Review)
(18 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, PLA, WB
NOD2 Antibody
(2D9) - BSA Free
NB100-524
Flow Cytometry NOD2 Antibody (2D9) - BSA Free
Flow Cytometry NOD2 Antibody (2D9) - BSA Free
Immunohistochemistry-Frozen NOD2 Antibody (2D9) - BSA Free
(1 Review)
(26 Publications)
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
MCAT Antibody (2F2)
H00027349-M01
Immunohistochemistry-Paraffin MCAT Antibody (2F2)
Western Blot MCAT Antibody (2F2)
Sandwich ELISA MCAT Antibody (2F2)
Species: Hu
Applications: ELISA, IHC, IHC-P, S-ELISA, WB
Metallothionein-2A
Antibody (6G2)
H00004502-M01
ELISA Metallothionein-2A Antibody (6G2)
Immunocytochemistry/ Immunofluorescence Metallothionein-2A Antibody (6G2)
(3 Publications)
Species: Hu, Mu
Applications: ELISA, ICC/IF, IHC, WB
HLA DQ/DR/DP
Antibody (HLA-Pan/296 ...
NBP2-79843
Protein Array HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Western Blot HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
PMEL17/SILV
Antibody (HMB45)
NBP2-44520
Flow (Intracellular) PMEL17/SILV Antibody (HMB45)
Flow (Intracellular) PMEL17/SILV Antibody (HMB45)
Immunohistochemistry PMEL17/SILV Antibody (HMB45)
(8 Publications)
Species: Ca(-), Hu, Rt(-)
Applications: Flow-IC, Flow, ICC/IF, IF, IHC, IHC-P, MI, WB

Melanotransferrin/CD22 ...
MAB8175
Melanotransferrin/CD228/MFI2 Antibody (893438) [Unconjugated]
Species: Hu
Applications: WB, Simple Western, IHC

Publications for Melanotransferrin/CD228/MFI2 Antibody (MAB8175) (0)

There are no publications for Melanotransferrin/CD228/MFI2 Antibody (MAB8175).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Melanotransferrin/CD228/MFI2 Antibody (MAB8175) (0)

There are no reviews for Melanotransferrin/CD228/MFI2 Antibody (MAB8175). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Melanotransferrin/CD228/MFI2 Antibody (MAB8175) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Melanotransferrin/CD228/MFI2 Products

Blogs on Melanotransferrin/CD228/MFI2

There are no specific blogs for Melanotransferrin/CD228/MFI2, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Flow Cytometry: NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on Jurkat cells with NOD2 (2D9) antibody NB100-524APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes.
NOD2 Antibody (2D9) - BSA Free
NB100-524

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Melanotransferrin/CD228/MFI2 Antibody (893438) [Unconjugated] and receive a gift card or discount.


Bioinformatics

Gene Symbol MFI2
Uniprot