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Argininosuccinate Lyase Antibody (OTI3D4) - Azide and BSA Free

Images

 
Western Blot: Argininosuccinate Lyase Antibody (OTI3D4) - Azide and BSA Free [NBP2-70213] - Analysis of HT29 cell lysate (35ug).
Immunohistochemistry: Argininosuccinate Lyase Antibody (OTI3D4) - Azide and BSA Free [NBP2-70213] - Analysis of Carcinoma of Human liver tissue.
Western Blot: Argininosuccinate Lyase Antibody (OTI3D4) - Azide and BSA Free [NBP2-70213] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Argininosuccinate Lyase.
Immunohistochemistry: Argininosuccinate Lyase Antibody (OTI3D4) - Azide and BSA Free [NBP2-70213] - Analysis of Human liver tissue.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI3D4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

Argininosuccinate Lyase Antibody (OTI3D4) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human ASL (NP_001020114) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ASL
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
Theoretical MW
51.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Argininosuccinate Lyase Antibody (OTI3D4) - Azide and BSA Free

  • argininosuccinase
  • argininosuccinate lyase
  • arginosuccinase
  • ASAL
  • EC 4.3.2.1

Background

Argininosuccinate Lyase encodes a member of the lyase 1 family. The encoded protein forms a cytosolic homotetramer and primarily catalyzes the reversible hydrolytic cleavage of argininosuccinate into arginine and fumarate, an essential step in the liver in detoxifying ammonia via the urea cycle. Mutations in this gene result in the autosomal recessive disorder argininosuccinic aciduria, or argininosuccinic acid lyase deficiency. A nontranscribed pseudogene is also located on the long arm of chromosome 22. Alternatively spliced transcript variants encoding different isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ASL