![Western Blot: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - Blot showing the effect of hypoxia on the protein expression levels of the purine biosynthetic enzymes. HIF-1 alpha is stabilized in hypoxia as expected, and no significant increase in the purine enzymes was detected between normoxic (21% oxygen) and hypoxic (1% oxygen) growth conditions. The positions of molecular markers surrounding each band of interest are shown for each blot. ADSL (NBP2-03107), ATIC (NBP2-01941), FGAMS (NBP1-84691), GART (H00002618-M01), HIF-1a (NB100-449), PAICS (NBP2-02817), PPAT (NBP2-02056). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32439803/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-OTI2D10-Western-Blot-NBP2-03107-img0012.jpg "Western Blot: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - Blot showing the effect of hypoxia on the protein expression levels of the purine biosynthetic enzymes. HIF-1 alpha is stabilized in hypoxia as expected, and no significant increase in the purine enzymes was detected between normoxic (21% oxygen) and hypoxic (1% oxygen) growth conditions. The positions of molecular markers surrounding each band of interest are shown for each blot. ADSL (NBP2-03107), ATIC (NBP2-01941), FGAMS (NBP1-84691), GART (H00002618-M01), HIF-1a (NB100-449), PAICS (NBP2-02817), PPAT (NBP2-02056). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32439803/) licensed under a CC-BY license.")
| Reactivity | Hu, Mu, Rt, Ca, Pm, PmSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | OTI2D10 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.55 mg/ml |
| Immunogen | Full length human recombinant protein of human ADSL (NP_000017) produced in HEK293T cell. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ADSL |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 54.7 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.55 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Adenylosuccinate Lyase Antibody (NBP2-03107)Find related products by research area.
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![Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - Staining of paraffin-embedded Human lymphoma tissue using anti-Adenylosuccinate Lyase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-OTI2D10-Immunohistochemistry-Paraffin-NBP2-03107-img0010.jpg "Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - Staining of paraffin-embedded Human lymphoma tissue using anti-Adenylosuccinate Lyase mouse monoclonal antibody.")
![Flow Cytometry: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Adenylosuccinate Lyase antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-OTI2D10-Flow-Cytometry-NBP2-03107-img0001.jpg "Flow Cytometry: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Adenylosuccinate Lyase antibody, and then analyzed by flow cytometry.")
![Western Blot: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Adenylosuccinate Lyase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Adenylosuccinate Lyase.](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-OTI2D10-Western-Blot-NBP2-03107-img0002.jpg "Western Blot: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Adenylosuccinate Lyase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Adenylosuccinate Lyase.")
![Western Blot: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Adenylosuccinate Lyase monoclonal antibody.](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-OTI2D10-Western-Blot-NBP2-03107-img0011.jpg "Western Blot: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Adenylosuccinate Lyase monoclonal antibody.")
![Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Adenylosuccinate Lyase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-OTI2D10-Immunohistochemistry-Paraffin-NBP2-03107-img0003.jpg "Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Adenylosuccinate Lyase mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Adenylosuccinate Lyase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-OTI2D10-Immunohistochemistry-Paraffin-NBP2-03107-img0004.jpg "Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Adenylosuccinate Lyase mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-Adenylosuccinate Lyase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-OTI2D10-Immunohistochemistry-Paraffin-NBP2-03107-img0005.jpg "Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-Adenylosuccinate Lyase mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-Adenylosuccinate Lyase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-OTI2D10-Immunohistochemistry-Paraffin-NBP2-03107-img0006.jpg "Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-Adenylosuccinate Lyase mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Adenylosuccinate Lyase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-OTI2D10-Immunohistochemistry-Paraffin-NBP2-03107-img0007.jpg "Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Adenylosuccinate Lyase mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - Staining of paraffin-embedded Human Kidney tissue using anti-Adenylosuccinate Lyase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-OTI2D10-Immunohistochemistry-Paraffin-NBP2-03107-img0008.jpg "Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - Staining of paraffin-embedded Human Kidney tissue using anti-Adenylosuccinate Lyase mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - Staining of paraffin-embedded Human liver tissue using anti-Adenylosuccinate Lyase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Adenylosuccinate-Lyase-Antibody-OTI2D10-Immunohistochemistry-Paraffin-NBP2-03107-img0009.jpg "Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - Staining of paraffin-embedded Human liver tissue using anti-Adenylosuccinate Lyase mouse monoclonal antibody.")
![Immunohistochemistry CFTR Antibody (24-1) [Unconjugated] - C-terminus](https://images.novusbio.com/images2/CFTR_MAB25031_Immunohistochemistry_6973.jpg)
![Immunohistochemistry CFTR Antibody (24-1) [Unconjugated] - C-terminus](https://images.novusbio.com/images2/CFTR_MAB25031_Immunohistochemistry_9671.jpg)
![Immunoprecipitation CFTR Antibody (24-1) [Unconjugated] - C-terminus](https://images.novusbio.com/images/mab25031_human-cftr-c-terminus-mab-clone-24-1-41202412513815.jpg)
![Western Blot Prostatic Acid Phosphatase/ACPP Antibody [Unconjugated]](https://images.novusbio.com/images2/Prostatic_Acid_Phosphatase_AF6240_Western_Blot_10680.jpg)
![Immunohistochemistry Prostatic Acid Phosphatase/ACPP Antibody [Unconjugated]](https://images.novusbio.com/images2/Prostatic_Acid_Phosphatase_AF6240_Immunohistochemistry_10732.jpg)
![ELISA Prostatic Acid Phosphatase/ACPP Antibody [Unconjugated]](https://images.novusbio.com/images2/Prostatic_Acid_Phosphatase_AF6240_ELISA_22233.jpg)
![Bioactivity GM-CSF [Unconjugated]](https://images.novusbio.com/images/protein/7954-gmcf_recombinant-human-gm-csf-cho-expressed-protein-cf-bioactivity-272020121514.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")