Home » ARC/NOL3 » ARC/NOL3 Lysates » ARC/NOL3 Overexpression Lysate
Novus Biologicals products are now on bio-techne.com

ARC/NOL3 Overexpression Lysate

 Submit a Review

Images

 
(
Enlarge)
Western Blot: ARC/NOL3 Overexpression Lysate [NBL1-13710] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NOL3.
Western Blot: ARC/NOL3 Overexpression Lysate [NBL1-13710] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NOL3.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

ARC/NOL3 Overexpression Lysate Summary

Description

ARC/NOL3 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC200591

Accession#: NM_003946

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
NOL3

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
22.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for ARC/NOL3 Overexpression Lysate

  • Apoptosis repressor with CARD
  • ARC
  • ARCFLJ35304
  • CARD2
  • Muscle-enriched cytoplasmic protein
  • MYC
  • MYP
  • NOL3
  • NOP30
  • NOPnucleolar protein 3
  • nucleolar protein 3 (apoptosis repressor with CARD domain)
  • Nucleolar protein of 30 kDa

Background

Nop30 (nucleolar protein of 30 kDa) is predominantly expressed in muscle and is present in the nucleoplasm and concentrated in nucleoli. The predominant nucleolar localization of Nop30 points to a potential role in RNA processing in muscle nucleoli. A CARD domain containing protein, designated ARC for apoptosis repressor with CARD is related to Nop30 with a different carboxyl terminus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Neuropeptide Y
Antibody
NBP1-46535
Immunohistochemistry Neuropeptide Y Antibody
Immunocytochemistry/ Immunofluorescence Neuropeptide Y Antibody
(20 Publications)
Species: Hu, Mu, Rb, Rt, Xp
Applications: ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, WB
POMC Antibody
NB100-1533
Flow Cytometry POMC Antibody
Immunohistochemistry POMC Antibody
Immunohistochemistry POMC Antibody
(10 Publications)
Species: Hu, Mu, Po, Rt, Sh
Applications: Flow, ICC/IF, IHC, IHC-P, PEP-ELISA, WB
c-Fos Antibody
(2H2)
NBP2-50037
Knockout Validated c-Fos Antibody (2H2)
Immunocytochemistry/ Immunofluorescence c-Fos Antibody (2H2)
Immunocytochemistry/ Immunofluorescence c-Fos Antibody (2H2)
(1 Review)
(19 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, KO, WB
Nociceptin Antibody
NBP2-58314
Immunohistochemistry-Paraffin Nociceptin Antibody
Immunohistochemistry-Paraffin Nociceptin Antibody
Immunohistochemistry-Paraffin Nociceptin Antibody
Species: Hu
Applications: IHC, IHC-P
LIGHT/TNFSF14
[Unconjugated]
664-LI
SDS-PAGE LIGHT/TNFSF14 [Unconjugated]
Bioactivity LIGHT/TNFSF14 [Unconjugated]
(21 Publications)
Species: Hu
Applications: BA
Caspase-3 Antibody
[Unconjugated] - Acti ...
AF835
Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active
Immunohistochemistry Caspase-3 Antibody [Unconjugated] - Active
Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active
(7 Reviews)
(383 Publications)
Species: Hu, Mu
Applications: ICC, IHC
Leptin/OB [HRP]
DLP00
N/A Leptin/OB [HRP]
N/A Leptin/OB [HRP]
(150 Publications)
Species: Hu
Applications: ELISA
Bcl-2 Antibody
NB100-56098
Western Blot Bcl-2 Antibody
Western Blot Bcl-2 Antibody
Western Blot Bcl-2 Antibody
(2 Reviews)
(26 Publications)
Species: Ca, Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
BDNF [HRP]
DBD00
N/A BDNF [HRP]
N/A BDNF [HRP]
N/A BDNF [HRP]
(100 Publications)
Species: Hu
Applications: ELISA
ORL1/OPRL1 Antibody
NBP1-69143
Western Blot ORL1/OPRL1 Antibody
Western Blot ORL1/OPRL1 Antibody
Western Blot ORL1/OPRL1 Antibody
(1 Publication)
Species: Hu
Applications: WB
CD4 Antibody - BSA
Free
NBP1-19371
Immunohistochemistry-Frozen CD4 Antibody - BSA Free
Immunohistochemistry-Paraffin CD4 Antibody - BSA Free
Flow Cytometry CD4 Antibody - BSA Free
(9 Reviews)
(50 Publications)
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(803 Publications)
Species: Hu
Applications: BA
EGR1 Antibody
[Unconjugated]
AF2818
EGR1 Antibody [Unconjugated]
(13 Publications)
Species: Hu
Applications: ICC, WB
Insulin Antibody
(182410) [Unconjugate ...
MAB1417
Immunocytochemistry Insulin Antibody (182410) [Unconjugated]
Immunohistochemistry Insulin Antibody (182410) [Unconjugated]
(24 Publications)
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
GRIP1 Antibody -
BSA Free
NBP2-41211
Western Blot GRIP1 Antibody - BSA Free
Immunohistochemistry GRIP1 Antibody - BSA Free
Immunohistochemistry GRIP1 Antibody - BSA Free
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
Bax Antibody (6A7)
NBP1-28566
Western Blot Bax Antibody (6A7)
Western Blot Bax Antibody (6A7)
Immunohistochemistry-Frozen Bax Antibody (6A7)
(3 Reviews)
(20 Publications)
Species: Bv, Hu, Pm, Mu, Po, Rt
Applications: Func, IHC, IHC-Fr, IHC-P, WB
Catalase Antibody
[Unconjugated]
AF3398
Western Blot Catalase Antibody [Unconjugated]
Simple Western Catalase Antibody [Unconjugated]
Knockout Validated Catalase Antibody [Unconjugated]
(3 Reviews)
(15 Publications)
Species: Hu, Mu, Rt
Applications: ICC, KO, Simple Western, WB
AgRP/ART [HRP]
DAGR00
N/A AgRP/ART [HRP]
N/A AgRP/ART [HRP]
N/A AgRP/ART [HRP]
(3 Publications)
Species: Hu
Applications: ELISA

Publications for ARC/NOL3 Lysate (NBL1-13710) (0)

There are no publications for ARC/NOL3 Lysate (NBL1-13710).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for ARC/NOL3 Lysate (NBL1-13710) (0)

There are no reviews for ARC/NOL3 Lysate (NBL1-13710). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ARC/NOL3 Lysate (NBL1-13710) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional ARC/NOL3 Products

Research Areas for ARC/NOL3 Lysate (NBL1-13710)

Find related products by research area.

Blogs on ARC/NOL3

There are no specific blogs for ARC/NOL3, but you can read our latest blog posts.

Customers Who Bought This Also Bought

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005
Caspase‑3 was detected in immersion fixed anti-FAS treated Jurkat human acute T cell leukemia cell line using 0.3 µg/mL Human/Mouse Active Caspase‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF835) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Caspase-3 Antibody [Unconjugated] - Active
AF835

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ARC/NOL3 Overexpression Lysate and receive a gift card or discount.


Bioinformatics

Gene Symbol NOL3