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ARA54 Antibody (4G9)

Images

 
Western Blot: ARA54 Antibody (4G9) [H00009604-M01] - Analysis of RNF14 expression in transfected 293T cell line by RNF14 monoclonal antibody (M01), clone 4G9.Lane 1: RNF14 transfected lysate(39.6 KDa).Lane 2: ...read more
Immunocytochemistry/ Immunofluorescence: ARA54 Antibody (4G9) [H00009604-M01] - Analysis of monoclonal antibody to RNF14 on HeLa cell . Antibody concentration 10 ug/ml.
Western Blot: ARA54 Antibody (4G9) [H00009604-M01] - Analysis of RNF14 over-expressed 293 cell line, cotransfected with RNF14 Validated Chimera RNAi ( Cat # H00009604-R01V ) (Lane 2) or non-transfected control (Lane 1). ...read more
Sandwich ELISA: ARA54 Antibody (4G9) [H00009604-M01] - Detection limit for recombinant GST tagged RNF14 is approximately 0.1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, KD
Clone
4G9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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ARA54 Antibody (4G9) Summary

Immunogen
RNF14 (NP_004281, 217 a.a. ~ 316 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LFLCSICFCEKLGSECMYFLECRHVYCKACLKDYFEIQIRDGQVQCLNCPEPKCPSVATPGQVKELVEAELFARYDRLLLQSSLDLMADVVYCPRPCCQL
Specificity
RNF14 - ring finger protein 14
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
RNF14
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Knockdown Validated
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactive against recombinant protein for western blot. It has also been used for RNAi validation and ELISA and immunofluorescence.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ARA54 Antibody (4G9)

  • Androgen receptor-associated protein 54
  • ARA54
  • ARA54androgen receptor associated protein 54
  • EC 6.3.2.-
  • HFB30
  • HFB30E3 ubiquitin-protein ligase RNF14
  • ring finger protein 14FLJ26004
  • RNF14
  • Triad2 protein
  • TRIAD2

Background

The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of this gene has been demonstrated to inhibit the AR-mediated growth of prostate cancer. This protein also interacts with class III ubiquitin-conjugating enzymes (E2s) and may act as a ubiquitin-ligase (E3) in the ubiquitination of certain nuclear proteins. Five alternatively spliced transcript variants encoding two distinct isoforms have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol RNF14
Entrez
OMIM
Uniprot