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APAF-1 Antibody (2H9A1) - BSA Free

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Western Blot: APAF-1 Antibody (2H9A1) [NBP2-61868] - Analysis using APAF1 mAb against human APAF1 (AA: 1138-1237) recombinant protein. (Expected MW is 37 kDa)
Western Blot: APAF-1 Antibody (2H9A1) [NBP2-61868] - Analysis using APAF1 mAb against human APAF1 (AA: 1138-1237) recombinant protein. (Expected MW is 37 kDa)
Immunocytochemistry/ Immunofluorescence: APAF-1 Antibody (2H9A1) [NBP2-61868] - Analysis of Hela cells using APAF1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa ...read more
Flow Cytometry: APAF-1 Antibody (2H9A1) [NBP2-61868] - Analysis of Hela cells using APAF1 mouse mAb (green) and negative control (red).
Western Blot: APAF-1 Antibody (2H9A1) [NBP2-61868] - Analysis using APAF1 mAb against HEK293 (1) and APAF1 (AA: 1138-1237)-hIgGFc transfected HEK293 (2) cell lysate.
ELISA: APAF-1 Antibody (2H9A1) [NBP2-61868] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clone
2H9A1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP2-61868-0.025ml
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APAF-1 Antibody (2H9A1) - BSA Free Summary

Immunogen
Purified recombinant fragment of human APAF-1 (AA: 1138-1237) expressed in E. Coli.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
APAF1
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Western Blot 1:500-1:2000
Theoretical MW
141.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for APAF-1 Antibody (2H9A1) - BSA Free

  • APAF1
  • APAF-1
  • APAF-1apoptotic protease activating factor 1
  • apoptotic peptidase activating factor 1
  • apoptotic peptidase activating factor
  • apoptotic protease activating factor
  • apoptotic protease-activating factor 1
  • CED4
  • DKFZp781B1145
  • KIAA0413

Background

This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-61868
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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol APAF1