FLASH Antibody - BSA Free Summary
Immunogen |
N-terminus of the FLASH protein. |
Localization |
Cytoplasmic and Nuclear |
Specificity |
Reacts with the N terminus of the FLASH protein. |
Isotype |
IgG |
Clonality |
Polyclonal |
Host |
Rabbit |
Gene |
CASP8AP2 |
Purity |
Immunogen affinity purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Western Blot 1:500 -1:1000
|
Application Notes |
Western blot - Use at 1:500 to 1:1,000 dilution. However, the investigator should determine the optimal dilution for a specific application such as immunohistochemistry.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Theoretical MW |
212 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Reactivity Notes
Human, Mouse. Others species have not been tested.
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
PBS |
Preservative |
0.02% Sodium Azide |
Concentration |
1 mg/ml |
Purity |
Immunogen affinity purified |
Alternate Names for FLASH Antibody - BSA Free
Background
Fas is a cell-surface receptor molecule that relays apoptotic signals into cells. When Fas is activated by the binding of its ligand, the proteolytic protein caspase-8 is recruited to a signaling complex known as DISC by binding to a Fas-associated adapter protein. A new protein, FLASH, contains a motif with oligomerizing activity whose sequence is similar to that of the Caenorhabditis elegans protein CED-4, and another domain (DRD domain) that interacts with a death-effector domain in caspase-8 or in the adapter protein. FLASH is likely a component of the DISC signaling complex and is necessary for the activation of caspase-8 in Fas-mediated apoptosis.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
Video Protocols
FAQs for FLASH Antibody (NB500-218). (Showing 1 - 1 of 1 FAQ).
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Would you be able to confirm the immunogen and epitopes for NB500-218, NB500-219 - FLASH Antibodies?
- NB500-219 was made to the C-terminal region of the FLASH protein and will recognize an epitope in this region. Product NB500-218 is made to the N-terminal region and will recognize an epitope in the N-terminal region.
Secondary Antibodies
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Isotype Controls
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