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AP3M2 Antibody

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Western Blot: AP3M2 Antibody [NBP2-15419] - Sample (30 ug of whole cell lysate) A: U87-MG 12% SDS PAGE; antibody diluted at 1:1000.
Western Blot: AP3M2 Antibody [NBP2-15419] - Sample (30 ug of whole cell lysate) A: U87-MG 12% SDS PAGE; antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: AP3M2 Antibody [NBP2-15419] - Human hepatoma, using AP3M2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, Xp, ZeSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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AP3M2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human AP3M2. The exact sequence is proprietary.
Localization
Cell membrane; Membrane; coated pit
Predicted Species
Mouse (98%), Rat (98%), Canine (100%), Xenopus (94%), Zebrafish (96%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AP3M2
Purity
Antigen Affinity-purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (94%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for AP3M2 Antibody

  • Adapter-related protein complex 3 mu-2 subunit
  • adaptor-related protein complex 3, mu 2 subunit
  • AP-3 complex subunit mu-2
  • AP47B
  • CLA20
  • Clathrin assembly protein assembly protein complex 1 medium chain homolog 2
  • Clathrin coat assembly protein AP47 homolog 2
  • Clathrin coat-associated protein AP47 homolog 2
  • clathrin-associated protein AP47 homolog 2
  • Golgi adaptor AP-1 47 kDa protein homolog 2
  • HA1 47 kDa subunit homolog 2
  • HA1 47kDA subunit homolog 2
  • mu3B-adaptin
  • P47B

Background

This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. The encoded protein may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol AP3M2
Uniprot