![Western Blot: AP2S1 Antibody (3E4) [H00001175-M01] - Analysis of AP2S1 expression in transfected 293T cell line by AP2S1 monoclonal antibody (M01), clone 3E4.Lane 1: AP2S1 transfected lysate(17 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/AP2S1-Antibody-3E4-Western-Blot-H00001175-M01-img0005.jpg "Western Blot: AP2S1 Antibody (3E4) [H00001175-M01] - Analysis of AP2S1 expression in transfected 293T cell line by AP2S1 monoclonal antibody (M01), clone 3E4.Lane 1: AP2S1 transfected lysate(17 KDa).Lane 2: Non-transfected lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, KD |
| Clone | 3E4 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Quality control test: Antibody Reactive Against Recombinant Protein. |
| Immunogen | AP2S1 (AAH06337, 1 a.a. ~ 142 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MIRFILIQNRAGKTRLAKWYMQFDDDEKQKLIEEVHAVVTVRDAKHTNFVEFRNFKIIYRRYAGLYFCICVDVNDNNLAYLEAIHNFVEVLNEYFHNVCELDLVFNFYKVYTVVDEMFLAGEIRETSQTKVLKQLLMLQSLE |
| Specificity | AP2S1 (3E4) |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | AP2S1 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA and RNAi Validation. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for AP2S1 Antibody (H00001175-M01)Find related products by research area.
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![Western Blot: AP2S1 Antibody (3E4) [H00001175-M01] - Analysis of AP2S1 over-expressed 293 cell line, cotransfected with AP2S1 Validated Chimera RNAi ( Cat # H00001175-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with AP2S1 monoclonal antibody (M01), clone 3E4 (Cat # H00001175-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/AP2S1-Antibody-3E4-Western-Blot-H00001175-M01-img0003.jpg "Western Blot: AP2S1 Antibody (3E4) [H00001175-M01] - Analysis of AP2S1 over-expressed 293 cell line, cotransfected with AP2S1 Validated Chimera RNAi ( Cat # H00001175-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with AP2S1 monoclonal antibody (M01), clone 3E4 (Cat # H00001175-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.")
![Sandwich ELISA: AP2S1 Antibody (3E4) [H00001175-M01] - Detection limit for recombinant GST tagged AP2S1 is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/AP2S1-Antibody-3E4-Sandwich-ELISA-H00001175-M01-img0004.jpg "Sandwich ELISA: AP2S1 Antibody (3E4) [H00001175-M01] - Detection limit for recombinant GST tagged AP2S1 is 0.3 ng/ml as a capture antibody.")
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