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AP2M1 Antibody (OTI1E9) [DyLight 680]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI1E9
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 680

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-70417AF350
Alexa Fluor 405
NBP2-70417AF405
Alexa Fluor 488
NBP2-70417AF488
Alexa Fluor 532
NBP2-70417AF532
Alexa Fluor 594
NBP2-70417AF594
Alexa Fluor 647
NBP2-70417AF647
Alexa Fluor 700
NBP2-70417AF700
Alexa Fluor 750
NBP2-70417AF750
Allophycocyanin
NBP2-70417APC
Biotin
NBP2-70417B
CoraFluor 1
NBP2-70417CL1
DyLight 350
NBP2-70417UV
DyLight 405
NBP2-70417V
DyLight 488
NBP2-70417G
DyLight 550
NBP2-70417R
DyLight 594
NBP2-70417DL594
DyLight 650
NBP2-70417C
DyLight 755
NBP2-70417IR
FITC
NBP2-70417F
HRP
NBP2-70417H
Janelia Fluor 525
NBP2-70417JF525
Janelia Fluor 549
NBP2-70417JF549
Janelia Fluor 585
NBP2-70417JF585
Janelia Fluor 635
NBP2-70417JF635
Janelia Fluor 646
NBP2-70417JF646
Janelia Fluor 669
NBP2-70417JF669
PE
NBP2-70417PE
PerCP
NBP2-70417PCP
Unconjugated
NBP2-00834
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-00834
Azide and BSA Free
NBP2-70417
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AP2M1 Antibody (OTI1E9) [DyLight 680] Summary

Immunogen
Recombinant protein fragment corresponding to amino acids 97-383 of human AP2M1 (NP_001020376) produced in E.coli.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
AP2M1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for AP2M1 Antibody (OTI1E9) [DyLight 680]

  • Adaptin-mu2
  • adaptor-related protein complex 2, mu 1 subunit
  • AP-2 mu chain
  • Clathrin assembly protein complex 2 medium chain
  • clathrin coat adaptor protein AP50
  • Clathrin coat assembly protein AP50
  • Clathrin coat-associated protein AP50
  • clathrin-associated/assembly/adaptor protein, medium 1
  • HA2 50 kDa subunit
  • KIAA0109
  • mu subunit
  • Plasma membrane adaptor AP-2 50 kDa protein
  • plasma membrane adaptor AP-2 50kDA protein

Background

AP2M1 is a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. It is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. It may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Antibody (11H9) - BSA ...
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Immunocytochemistry/ Immunofluorescence Complement C3 Antibody (11H9) - BSA Free
Immunohistochemistry-Paraffin Complement C3 Antibody (11H9) - BSA Free
(2 Reviews)
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Immunohistochemistry-Paraffin AP3M2 Antibody
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(2H2)
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Immunocytochemistry/ Immunofluorescence c-Fos Antibody (2H2)
Immunocytochemistry/ Immunofluorescence c-Fos Antibody (2H2)
(1 Review)
(20 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, KO, WB
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[Unconjugated]
AF2214
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Immunohistochemistry FosB/G0S3 Antibody [Unconjugated]
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Western Blot JunD Antibody (512709)
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AP1M2 Antibody -
BSA Free
NBP2-92832
Western Blot AP1M2 Antibody - BSA Free
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Complement C4
Antibody
NBP3-12295
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Immunohistochemistry-Paraffin Complement C4 Antibody
Immunohistochemistry-Paraffin Complement C4 Antibody
Species: Hu, Pm, Rt
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AP1M1 Antibody -
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NBP2-92000
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NBP1-89985
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(2D9) - BSA Free
NB100-524
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Flow Cytometry NOD2 Antibody (2D9) - BSA Free
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(1 Review)
(26 Publications)
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol AP2M1