![Western Blot: Amphiphysin/AMPH Antibody [NB100-40788] - Western blot analysis of Amphiphysin using NB100-40788 at 0.5ug/ml in Mouse Brain lysate (35ug protein in RIPA buffer).](http://images.novusbio.com/fullsize/Amphiphysin-AMPH-Antibody-Western-Blot-NB100-40788-img0006.jpg "Western Blot: Amphiphysin/AMPH Antibody [NB100-40788] - Western blot analysis of Amphiphysin using NB100-40788 at 0.5ug/ml in Mouse Brain lysate (35ug protein in RIPA buffer).")
| Reactivity | Hu, Mu, Rt, CaSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-KRKDESRISKAE corresponding to internal region according to NP_001626.1, NP_647477.1. |
| Specificity | This antibody is expected to recognise both reported isoforms (NP_001626.1 and NP_647477.1). |
| Predicted Species | Rat (100%), Canine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | AMPH |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | WB: Approx. 125 kDa band observed in mouse brain lysates is reported in scientific literature (PMID: 9348539).
A minor band of unknown identity was also consistently observed at 45 kDa. This band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human cerebellum shows nuclear and fibrous staning. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
![Flow Cytometry ERMAP Antibody [Unconjugated]](https://images.novusbio.com/images2/ERMAP_AF4928_Flow_Cytometry_11957.jpg)
| Publication using NB100-40788 | Applications | Species |
|---|---|---|
| Sommer C, Weishaupt A, Brinkhoff J et al. Paraneoplastic stiff-person syndrome: passive transfer to rats by means of IgG antibodies to amphiphysin. Lancet 2005-04-01 [PMID: 15836889] |
Secondary Antibodies |
Isotype Controls |
Research Areas for Amphiphysin/AMPH Antibody (NB100-40788)Find related products by research area.
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| Gene Symbol | AMPH |
| Entrez | |
| Uniprot |
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![Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NB100-40788] - (2.5ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Amphiphysin-AMPH-Antibody-Immunohistochemistry-Paraffin-NB100-40788-img0005.jpg "Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NB100-40788] - (2.5ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
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