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Recombinant Human alpha-Fetoprotein/AFP His Protein

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SDS-Page: Recombinant Human alpha-Fetoprotein/AFP Protein [NBP2-52171] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain
SDS-Page: Recombinant Human alpha-Fetoprotein/AFP Protein [NBP2-52171] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.5 mg/ml

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Recombinant Human alpha-Fetoprotein/AFP His Protein Summary

Description
A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 19-609 of Human alpha-Fetoprotein/AFP

Source:Baculovirus

Amino Acid Sequence: ADPRTLHRNE YGIASILDSY QCTAEISLAD LATIFFAQFV QEATYKEVSK MVKDALTAIE KPTGDEQSSG CLENQLPAFL EELCHEKEIL EKYGHSDCCS QSEEGRHNCF LAHKKPTPAS IPLFQVPEPV TSCEAYEEDR ETFMNKFIYE IARRHPFLYA PTILLWAARY DKIIPSCCKA ENAVECFQTK AATVTKELRE SSLLNQHACA VMKNFGTRTF QAITVTKLSQ KFTKVNFTEI QKLVLDVAHV HEHCCRGDVL DCLQDGEKIM SYICSQQDTL SNKITECCKL TTLERGQCII HAENDEKPEG LSPNLNRFLG DRDFNQFSSG EKNIFLASFV HEYSRRHPQL AVSVILRVAK GYQELLEKCF QTENPLECQD KGEEELQKYI QESQALAKRS CGLFQKLGEY YLQNAFLVAY TKKAPQLTSS ELMAITRKMA ATAATCCQLS EDKLLACGEG AADIIIGHLC IRHEMTPVNP GVGQCCTSSY ANRRPCFSSL VVDETYVPPA FSDDKFIFHK DLCQAQGVAL QTMKQEFLIN LVKQKPQITE EQLEAVIADF SGLLEKCCQG QEQEVCFAEE GQKLISKTRA ALGVHHHHHH
Source
Baculovirus
Protein/Peptide Type
Recombinant Protein
Gene
AFP
Purity
>95%, by SDS-PAGE
Endotoxin Note
< 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

Dilutions
  • SDS-Page
Theoretical MW
67.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 10% glycerol
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>95%, by SDS-PAGE

Alternate Names for Recombinant Human alpha-Fetoprotein/AFP His Protein

  • AFP
  • Alpha-1-fetoprotein
  • alpha-fetoglobulin
  • alpha-Fetoprotein
  • DSCAM2
  • FETA
  • HP
  • HPAFP

Background

Alpha 1 Fetoprotein encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol AFP