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Rat Alkaline Phosphatase, Intestinal ELISA Kit (Colorimetric)

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Rat Alkaline Phosphatase, Intestinal ELISA Kit (Colorimetric) [NBP2-68196] - Standard Curve Reference
ELISA: Rat Alkaline Phosphatase, Intestinal ELISA Kit (Colorimetric) [NBP2-68196] - Samples were spiked with high concentrations of Rat Alkaline Phosphatase, Intestinal and diluted with Reference Standard & Sample ...read more
ELISA: Rat Alkaline Phosphatase, Intestinal ELISA Kit (Colorimetric) [NBP2-68196] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.78 - 50 ng/mL
Sensitivity
0.47 ng/mL

Order Details

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Rat Alkaline Phosphatase, Intestinal ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat ALPI in samples. No significant cross-reactivity or interference between Rat ALPI and analogues was observed.
Standard Curve Range
0.78 - 50 ng/mL
Sensitivity
0.47 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.8%
Intra-Assay
CV% < 4.7%
Spike Recovery
88-106%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
ALPI

Applications/Dilutions

Dilutions
  • ELISA
Publications
Read Publications using NBP2-68196.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Rat Alkaline Phosphatase, Intestinal ELISA Kit (Colorimetric)

  • alkaline phosphatase, intestinal
  • Alkaline phosphomonoesterase
  • ALPI
  • EC 3.1.3.1
  • Glycerophosphatase
  • IAP
  • Intestinal alkaline phosphatase
  • intestinal-type alkaline phosphatase
  • Kasahara isozyme

Background

Alkaline phosphatase removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes - placental, placental-like, intestinal and non-tissue specific (found in liver, kidney and bone).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Publications for Alkaline Phosphatase, Intestinal ELISA Kit (NBP2-68196)(2)

We have publications tested in 1 application: ELISA.


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Showing Publications 1 - 2 of 2.
Publications using NBP2-68196 Applications Species
Wang Z, Jiang X, Zhang L, Chen H Protective effects of Althaea officinalis L. extract against N ?diethylnitrosamine?induced hepatocellular carcinoma in male Wistar rats through antioxidative, anti?inflammatory, mitochondrial apoptosis and PI3K /Akt/ mTOR signaling pathways Food Science & Nutrition 2023-05-26 (ELISA) ELISA
Zhenqian Wang, Xiao Jiang, Long Zhang, Han Chen Protective effects of Althaea officinalis L. extract against N ‐diethylnitrosamine‐induced hepatocellular carcinoma in male Wistar rats through antioxidative, anti‐inflammatory, mitochondrial apoptosis and PI3K /Akt/ mTOR signaling pathways Food Science & Nutrition 2023-05-26 [PMID: 37576045]

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Bioinformatics

Gene Symbol ALPI