ALK/CD246 Antibody (ALK/1032) [DyLight 405]

Recombinant fragment (around aa 220-335) of human ALK/CD246 protein (exact sequence is proprietary) (Uniprot: Q9UM73)

Cytoplasmic and Nuclear

The wild-type anaplastic lymphoma kinase (ALK) protein is a 200kDa transmembrane receptor tyrosine kinase. Its expression is restricted to a few scattered cells in the nervous system (some glial cells and neurons, and a few endothelial cells and pericytes. The hybrid gene,NPM-ALK, created by the t(2;5)(p23;q35) chromosomal translocation encodes part of the nucleolar phosphoprotein, nucleophosmin (NPM), joined to the entire cytoplasmic portion of the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. As a consequence, the ALK gene comes under the control of the NPM promoter, which induces a permanent and ubiquitous transcription of the NPM-ALK hybrid gene, resulting in the production of a 80kDa NPM-ALK chimeric protein. This translocation is found in anaplastic large cell lymphomas (ALCL). Reportedly, expression of ALK indicates a better prognosis. Approximately 5%-10% of non-small cell lung carcinomas also express ALK protein producing a cytoplasmic staining pattern. This monoclonal antibody also reacts with blood vessels that serves as an internal positive control.

IgG2b Kappa

Monoclonal

Mouse

ALK

Protein A or G purified

Test in a species/application not listed above to receive a full credit towards a future purchase.

Optimal dilution of this antibody should be experimentally determined.