![Western Blot: Aldolase C Antibody (E9) [NBP2-50056] - Right: Blots of rat brain lysate blotted with NBP2-50056. NBP2-50056 binds strongly and cleanly to a band at about 40 kDa. This antibody binds to an epitope shared by the closely related Aldolase A, B and C, recognizing all three on western blots. Above: Blots of recombinant human Aldolase A, B and C with NBP2-50056 as indicated showing binding to all three gene products. Lane labelled S show molecular weight standards, while lanes A, B and C contain recombinant full length His-tagged human Aldolase A, B and C respectively. The epitope for NBP2-50056 is within the core of the three Aldolase molecules which are less conserved than the N and C-termini where Aldolase C specific antibodies bind. The specificity of two other Aldolase antibodies is shown in the indicated parts of the image. One antibody binds to the N-terminal peptide (clone 4A9) and one to the C-terminal peptide (clone 1A1).](http://images.novusbio.com/fullsize/Aldolase-C-Antibody-E9-Western-Blot-NBP2-50056-img0004.jpg "Western Blot: Aldolase C Antibody (E9) [NBP2-50056] - Right: Blots of rat brain lysate blotted with NBP2-50056. NBP2-50056 binds strongly and cleanly to a band at about 40 kDa. This antibody binds to an epitope shared by the closely related Aldolase A, B and C, recognizing all three on western blots. Above: Blots of recombinant human Aldolase A, B and C with NBP2-50056 as indicated showing binding to all three gene products. Lane labelled S show molecular weight standards, while lanes A, B and C contain recombinant full length His-tagged human Aldolase A, B and C respectively. The epitope for NBP2-50056 is within the core of the three Aldolase molecules which are less conserved than the N and C-termini where Aldolase C specific antibodies bind. The specificity of two other Aldolase antibodies is shown in the indicated parts of the image. One antibody binds to the N-terminal peptide (clone 4A9) and one to the C-terminal peptide (clone 1A1).")
| Reactivity | Hu, Mu, Rt, Po, Bv, Ch, EqSpecies Glossary |
| Applications | WB, ICC/IF |
| Clone | E9 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Full length recombinant human Aldolase C. [UniProt# P09972] |
| Specificity | Also reacts with Aldolase A and B. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ALDOC |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 40 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 50% PBS, 50% glycerol |
| Preservative | 0.035% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Aldolase C Antibody (NBP2-50056)Find related products by research area.
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RNA-binding protein Staufen1 conspires with Atxn2 in stress granules to cause neurodegeneration by dysregulating RNA metabolism By Jamshed Arslan Pharm.D. Spinocerebellar ataxia type 2 (SCA2) is a movement disorder characterized by neurodegeneration. The cause of this autosomal dominant disease is a mutation in the RNA processing gene Atxn2,... Read full blog post. |
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![Immunocytochemistry/Immunofluorescence: Aldolase C Antibody (E9) [NBP2-50056] - View of mixed neuron/glial cultures stained with NBP2-50056 (red) and chicken antibody to GFAP (NBP1-05198, green). NBP2-50056 reveals strong cytoplasmic staining in astrocytes. Blue is a DNA stain. Aldolase stains the astrocytes cell body and processes, whereas GFAP labels the intermediate filament of the cytoskeleton in subset of astrocytes.](http://images.novusbio.com/fullsize/Aldolase-C-Antibody-E9-Immunocytochemistry-Immunofluorescence-NBP2-50056-img0003.jpg "Immunocytochemistry/Immunofluorescence: Aldolase C Antibody (E9) [NBP2-50056] - View of mixed neuron/glial cultures stained with NBP2-50056 (red) and chicken antibody to GFAP (NBP1-05198, green). NBP2-50056 reveals strong cytoplasmic staining in astrocytes. Blue is a DNA stain. Aldolase stains the astrocytes cell body and processes, whereas GFAP labels the intermediate filament of the cytoskeleton in subset of astrocytes.")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")