![Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NBP1-31378] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with AKR1C3 antibody diluted at 1:20000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Aldo-keto-Reductase-1C3-AKR1C3-Antibody-Western-Blot-NBP1-31378-img0013.jpg "Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NBP1-31378] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with AKR1C3 antibody diluted at 1:20000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
| Reactivity | Hu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Aldo-keto Reductase 1C3/AKR1C3. The exact sequence is proprietary. |
| Localization | Cytoplasm |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | AKR1C3 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 37 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 40% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Aldo-keto Reductase 1C3/AKR1C3 Antibody (NBP1-31378)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | AKR1C3 |
![Immunocytochemistry/Immunofluorescence: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NBP1-31378] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Aldo-keto-Reductase-1C3-AKR1C3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31378-img0006.jpg "Immunocytochemistry/Immunofluorescence: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NBP1-31378] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NBP1-31378] - NCI-N87 xenograft, using AKR1C3 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Aldo-keto-Reductase-1C3-AKR1C3-Antibody-Immunohistochemistry-Paraffin-NBP1-31378-img0007.jpg "Immunohistochemistry-Paraffin: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NBP1-31378] - NCI-N87 xenograft, using AKR1C3 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NBP1-31378] - Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Aldo-keto-Reductase-1C3-AKR1C3-Antibody-Western-Blot-NBP1-31378-img0008.jpg "Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NBP1-31378] - Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE; antibody diluted at 1:1000.")
![Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NBP1-31378] - A. 50 ug rat liver lysate/extract 10% SDS-PAGE AKR1C3 antibody [C2C3], C-term dilution: 1:2000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Aldo-keto-Reductase-1C3-AKR1C3-Antibody-Western-Blot-NBP1-31378-img0010.jpg "Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NBP1-31378] - A. 50 ug rat liver lysate/extract 10% SDS-PAGE AKR1C3 antibody [C2C3], C-term dilution: 1:2000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunoprecipitation: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NBP1-31378] - Immunoprecipitation of AKR1C3 protein from HepG2 whole cell extracts using 5 ug of AKR1C3 antibody [C2C3], C-term. Western blot analysis was performed using AKR1C3 antibody [C2C3], C-term. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Aldo-keto-Reductase-1C3-AKR1C3-Antibody-Immunoprecipitation-NBP1-31378-img0012.jpg "Immunoprecipitation: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NBP1-31378] - Immunoprecipitation of AKR1C3 protein from HepG2 whole cell extracts using 5 ug of AKR1C3 antibody [C2C3], C-term. Western blot analysis was performed using AKR1C3 antibody [C2C3], C-term. EasyBlot anti-Rabbit IgG was used as a secondary reagent.")
![Simple Western Aldo-keto Reductase 1C1/AKR1C1 Antibody (859026) [Unconjugated]](https://images.novusbio.com/images2/Aldoketo_Reductase_1C1_MAB6529_Simple_Western_17147.jpg)
![Western Blot Aldo-keto Reductase 1C1/AKR1C1 Antibody (859026) [Unconjugated]](https://images.novusbio.com/images2/Aldoketo_Reductase_1C1_MAB6529_Western_Blot_13068.jpg)
![N/A PLGF [HRP]](https://images.novusbio.com/images2/PlGF_DPG00_ELISA_158.jpg)
![N/A PLGF [HRP]](https://images.novusbio.com/images2/DATA_PlGF_DPG00_ELISA_788.jpg)
![N/A PLGF [HRP]](https://images.novusbio.com/images2/DATA_PlGF_DPG00_ELISA_789.jpg)
![Western Blot Androgen R/NR3C4 [p Ser213, p Ser210] Antibody (156C135.2) - BSA Free](https://images.novusbio.com/images/Androgen-R-NR3C4-p-Ser213-p-Ser210-Antibody-156C135-2-Western-Blot-NB100-56603-img0004.jpg)
![N/A Kallikrein 3/PSA [HRP]](https://images.novusbio.com/images2/DATA_Kallikrein_3_DKK300_ELISA_729.jpg)
![N/A Kallikrein 3/PSA [HRP]](https://images.novusbio.com/images2/DATA_Kallikrein_3_DKK300_ELISA_730.jpg)
![N/A Kallikrein 3/PSA [HRP]](https://images.novusbio.com/images2/Kallikrein_3_DKK300_ELISA_124.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")