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ALDH18A1 Antibody

Images

 
Independent Antibodies: Western Blot: ALDH18A1 Antibody [NBP1-83324] - Analysis using Anti-ALDH18A1 antibody NBP1-83324 (A) shows similar pattern to independent antibody NBP1-83325 (B).
Immunocytochemistry/ Immunofluorescence: ALDH18A1 Antibody [NBP1-83324] - Staining of human cell line A-431 shows localization to mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ALDH18A1 Antibody [NBP1-83324] - Staining of human gastrointestinal shows strong granular cytoplasmic positivity in glandular cells.
Western Blot: ALDH18A1 Antibody [NBP1-83324] - Analysis in human cell line HepG2.
Western Blot: ALDH18A1 Antibody [NBP1-83324] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: ALDH18A1 Antibody [NBP1-83324] - Staining of human placenta shows strong granular cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: ALDH18A1 Antibody [NBP1-83324] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
Immunohistochemistry-Paraffin: ALDH18A1 Antibody [NBP1-83324] - Staining of human liver shows no positivity in hepatocytes as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

       

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ALDH18A1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SVTFGTKSRVGMGGMEAKVKAALWALQGGTSVVIANGTHPKVSGHVITDIVEGKKVGTFFSEVKPAGPTVEQQGEMARSGGRMLATLEPEQRAEIIHHLADLLTDQRD
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ALDH18A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
ALDH18A1 Protein (NBP1-83324PEP)
Publications
Read Publications using
NBP1-83324 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ALDH18A1 Antibody

  • aldehyde dehydrogenase 18 family, member A1
  • Aldehyde dehydrogenase family 18 member A1
  • delta-1-pyrroline-5-carboxylate synthase
  • EC 2.1.2.11
  • EC 2.3.3.13
  • GSASdelta1-pyrroline-5-carboxlate synthetase
  • MGC117316
  • P5CSdelta-1-pyrroline-5-carboxylate synthetase
  • PYCSEC 1.2.1.41
  • pyrroline-5-carboxylate synthetase (glutamate gamma-semialdehyde synthetase)

Background

ALDH18A1, or Delta-1-pyrroline-5-carboxylate synthase, contains a long 88 kDa and a short 87 kDa isoform, and is involved in the catalytic conversion of glutamate to glutamate 5-semialdehyde. Current research on ALDH18A1 is being conducted to determine the relation between the protein and a variety of diseases and disorders, including neurodegeneration, pneumonia, connective tissue disease, mental retardation, hypoornithinemia, tuberculosis, microcephaly, Alzheimer's disease, gastroesophageal reflux disease, and dementia. The protein has been linked to biosynthesis and metabolic pathways where it interacts with PUS1, UQCRFS1, ICT1, OAT, and MYC.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ALDH18A1 Antibody (NBP1-83324)(4)

Reviews for ALDH18A1 Antibody (NBP1-83324) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ALDH18A1 Antibody (NBP1-83324). (Showing 1 - 1 of 1 FAQs).

  1. I am looking for mouse Aldh18a1 antibody suitable for immunohistochemistry, and find your antibodies NBP1-83324 and NBP1-83325. Do you have the data of WB or IHC using these antibodies against mouse samples? If not, I would like to test them in our samples. It would be great if you could give us an aliquot of them, and we will let you know the outcomes.
    • For our two products NBP1-83324 and NBP1-83325, we have not yet tested these products for detection of the mouse protein. I did run alignments of our antigens against the mouse sequence. Both sequences have a high homology to the mouse sequence. Since we have not yet tested these against mouse samples, you would qualify for our Innovator's Reward program. In exchange for a review of these products in your experiment, we would issue you a credit for the purchase price of the antibody. Regardless of results. Due to our Innovator's Reward Program, we do not offer free aliquots of our products. I am sorry for any inconvenience.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ALDH18A1
Entrez
Uniprot