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Rat ALAD ELISA Kit (Colorimetric)

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ELISA: Rat ALAD ELISA Kit (Colorimetric) [NBP2-68184] - Samples were spiked with high concentrations of Rat ALAD and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Rat ALAD ELISA Kit (Colorimetric) [NBP2-68184] - Samples were spiked with high concentrations of Rat ALAD and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of ...read more
ELISA: Rat ALAD ELISA Kit (Colorimetric) [NBP2-68184] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.78 - 50 ng/mL
Sensitivity
0.47 ng/mL

Order Details

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Rat ALAD ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat ALAD in samples. No significant cross-reactivity or interference between Rat ALAD and analogues was observed.
Standard Curve Range
0.78 - 50 ng/mL
Sensitivity
0.47 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.97%
Intra-Assay
CV% < 5.2%
Spike Recovery
88-106%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
ALAD

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Rat ALAD ELISA Kit (Colorimetric)

  • ALADHEC 4.2.1.24
  • aminolevulinate dehydratase
  • aminolevulinate, delta-, dehydratase
  • delta-aminolevulinic acid dehydratase
  • PBGSMGC5057
  • Porphobilinogen synthase

Background

The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins). ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway; zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product PDFs

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  Rat ALAD ELISA Kit (Colorimetric) Manual
  PDF Datasheet
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Bioinformatics

Gene Symbol ALAD