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Human AKR1C2 ELISA Kit (Colorimetric)

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ELISA: Human AKR1C2 ELISA Kit (Colorimetric) [NBP3-27336] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Tissue homogenates, cell lysates and other biological fluids
Standard Curve Range
0.156 - 10 ng/mL (example only; lot dependent)
Sensitivity
0.061 ng/mL (example only; lot dependent)

Order Details

Human AKR1C2 ELISA Kit (Colorimetric) Summary

Description
Assay Length: 4.5 hours
Standard Curve Range
0.156 - 10 ng/mL (example only; lot dependent)
Sensitivity
0.061 ng/mL (example only; lot dependent)
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 12 (example only; lot dependent)
Intra-Assay
%CV < 10 (example only; lot dependent)
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
AKR1C2

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Detection Reagent A
  2. Detection Reagent B
  3. Diluent Buffer
  4. Instruction manual
  5. Plate sealer for 96 wells
  6. Pre-coated 96T strip plate
  7. Standard
  8. Stop Solution
  9. TMB Substrate
  10. Wash Buffer (30 x concentrate)

Alternate Names for Human AKR1C2 ELISA Kit (Colorimetric)

  • aldo-keto reductase family 1 member C2
  • aldo-keto reductase family 1, member C2 (dihydrodiol dehydrogenase 2; bile acidbinding protein; 3-alpha hydroxysteroid dehydrogenase, type III)
  • BABP
  • Chlordecone reductase homolog HAKRD
  • DD
  • DD-2
  • DD2DD/BABP
  • DDH2FLJ53800
  • Dihydrodiol dehydrogenase 2
  • Dihydrodiol dehydrogenase/bile acid-binding protein
  • EC 1.1.1,3-alpha-HSD3
  • EC 1.1.1.213
  • EC 1.3.1.20
  • HAKRDAKR1C-pseudo
  • HBAB
  • MCDR2
  • pseudo-chlordecone reductase
  • Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase
  • type II dihydrodiol dehydrogenase
  • Type III 3-alpha-hydroxysteroid dehydrogenase

Background

AKR1C2 encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme binds bile acid with high affinity, and shows minimal 3-alpha-hydroxysteroid dehydrogenase activity. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol AKR1C2